NaCl-Induced Changes of Ion Fluxes in Roots of Transgenic Bacillus thuringiensis (Bt) Cotton (Gossypium hirsutum L.)

作     者：LI Mao-ying LI Fang-jun YUE Yue-sen TIAN Xiao-li LI Zhao-hu DUAN Liu-sheng 

作者机构：State Key Laboratory of Plant Physiology and Biochemistry Ministry of Education/Engineering Research Center of Plant Growth Regulator Ministry of Education/College of Agronomy and Biotechnology China Agricultural University 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2013年第12卷第3期

页      面：436-444页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the National Natural Science Foundation of China (30871490) the Specialized Research Fund for the Doctoral Program of Higher Education of China the Innovation Fund for Graduate Students of China Agricultural University (KYCX2011007) 

主　　题：transgenic Bt cotton salinity stress toxin protein SIET K^+ flux 

摘      要：Bacillus thuringiensis （Bt） cotton is grown worldwide, including in saline soils, but the effect of salinity on ion fluxes of Bt cotton remains unknown. Responses of two transgenic Bt cotton genotypes （SGK321 and 29317） and their corresponding receptors, Shiyuan 321 （SY321） and Jihe 321 （J321）, to 150 mmol L-1 NaCl stress were studied in a growth chamber. The root dry weight of SGK321 and 29317 under NaCl treatment was decreased by 30 and 31%, respectively. However, their corresponding receptor cultivars SY321 and J321 were less affected （19 and 24%, respectively）. The root length and surface area of the Bt cultivars were significantly decreased relative to their receptors under salt stress. NaCl treatment significantly increased CrylAc mRNA transcript levels in SGK321 and 29317 but did not affect Bt protein content in leaves or roots of either cultivar at 1 and 7 d after NaCl treatment. Fluxes of Na^＋, K^＋, and H^＋ in roots were investigated using the scanning ion-selective electrode technique. Both mean K^＋ efflux rate and transient K^＋ efflux of the Bt cultivars increased four-fold compared to their corresponding receptors when exposed to salinity stress. There were no significant differences in Na^＋ efflux between Bt and non-Bt cottons. Furthermore, the Na^＋ contents in roots and leaves of all genotypes dramatically increased under salt stress, whereas K^＋ contents decreased. Our results suggested that Bt cotton cultivars are more sensitive to salt stress than their receptor genotypes.