Detection of somatostatin mRNA in rat gastroentero-pancreatic system with digoxigenin-labeled cRNA probe in situ hybridization
Detection of somatostatin mRNA in rat gastroentero－pancreatic system with digoxigenin－labeled cRNA probe in situ hybridizatio出 版 物：《Journal of Medical Colleges of PLA(China)》 (中国人民解放军军医大学学报（英文版）)
年 卷 期：1996年第11卷第2期
页 面：139-141,146页
学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学]
主 题：digoxigenin somatostatin in situhybridization gastroenteropancreatic system cRNA probe rats
摘 要：Objective: To establish a sensitive, nonradioactive in situ hybridization method to detect the expression of gut regulatory peptide genes. Methods: The digoxigenin (Dig) labeled somatostatin (SS) RNA probes were synthesized with in vitro transcription system and the in situ hybridization was conducted on the cryostat sections of rat stomach , duodenum and pancreas. Results: Positive reaction sites were purple-blue in color and located in the cytoplasm with a light background and a strong contrast. Both the morphosis and distribution of the SS mRNA posrtive cells corresponded well with those of the previously reported SS immunoreactive cells. Conclusion: The Dig -labeled cRNA probe in situ hybridization method is simple, sensitive and reliable for the study of gut regulatory peptide gene expression.
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