Establishment of La-tPA/G-CSF dual transgenic mice and expression in their mammary gland

作     者：卢一凡 田靫 邓继先 程萱 黄培堂 

作者机构：1. Institute of Biotechnology Academy of Military Medicine Science 100071 Beijing China 2. Cardiovascular Institute Academy of Medicine Science 100037 Beijing China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：1999年第42卷第3期

页      面：330-336页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Project supported by the National High Technology Developmental Program "863" 

主　　题：La-tPA/G-CSF mammary double transgenic mice. 

摘      要：Expression vectors of human granulocyte colony stimulating factor (G-CSG ) and long acting tissue plas-minogen activator (La-tPA) in mammary gland were constructed using promoters of mouse whey acid protein gene (WAP) and sheep β-lactoglobulin gene (BLG) with sizes of 2.6 and 5 kb respectively. Two kinds of transgenic mice of G-CSF and La-tPA were produced with microinjection. The expression of G-CSF and La-tPA was achieved in mammary glands of transgenic mice, respectively. In order to establish dual transgenic mice of La-tPA /G-CSF, transgenic mice carrying G-CSF and La-tPA gene characterized with specific expression in mammary gland were mated. La-tPA/G-CSF dual transgenic mice were screened out from the hybrid offspring by Once-PCR. The co-expression of La-tPA and G-CSF in mammary gland of the dual transgenic mice was confirmed by the milk assayed and Northern blot analysis. Some parameters about the dual transgenic mice indicated that there were fewer litters than that of normal mice. The ratio of dual transgenes was 46.1 % in Fl generation, and offspring s sex ratio was normal. Hence a dual transgenic mouse model was established for the study of co-expression foreign proteins in mammary gland.