Chemical Protein Synthesis by Native Chemical Ligation and Variations Thereof

作     者：Siyao Wang Yogesh Abaso Thopate Qingqing Zhou Ping Wang 

作者机构：Shanghai Key Laboratory for Molecular Engineering of Chiral DrugsSchool of Chemistry and Chemical EngineeringShanghai Jiao Tong University800 Dongchuan RoadShanghai 200240China 

出 版 物：《Chinese Journal of Chemistry》 (中国化学（英文版）)

年 卷 期：2019年第37卷第11期

页      面：1181-1193页

核心收录：

学科分类：07[理学] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：Financial support for this work was provided by the National Natural Science Foundation of China(Nos.21672146 and 91753102) the Shanghai Committee of Science and Technol­ogy(No.17JC1405300). 

主　　题：processes. methods. synthesis 

摘      要：For a long time,the total synthesis of proteins was considered as a mission impossible because of the tedious and complex synthetic steps and de­manding purification processes.However,with the development of modern synthetic methodologies,many protein syntheses have now been reported.More importantly,through chemical synthesis,desired modifications can be installed to target proteins precisely,which is a major advantage over trad卜tional bio-synthesis approaches.This review summarizes the techniques developed for protein assembly,including native chemical ligation,5e-mediated ligation,and a range of other ligation methods. .