Downregulation of SNRPG induces cell cycle arrest and sensitizes human glioblastoma cells to temozolomide by targeting Myc through a p53-dependent signaling pathway

作     者：Yulong Lan Jiacheng Lou Jiliang Hu Zhikuan Yu Wen Lyu Bo Zhang Yulong Lan;Jiacheng Lou;Jiliang Hu;Zhikuan Yu;Wen Lyu;Bo Zhang

作者机构：Department of NeurosurgeryThe Second Affiliated Hospital of Dalian Medical UniversityDalian 116023China Department of NeurosurgeryShenzhen People's HospitalSecond Clinical Medical College of Jinan UniversityThe First Affiliated Hospital of Southern University of Science and TechnologyShenzhen 518020China 

出 版 物：《Cancer Biology & Medicine》 (癌症生物学与医学（英文版）)

年 卷 期：2020年第17卷第1期

页      面：112-131页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by grants from National Natural Science Foundation of China(Grant No.81372714,81672480,81872065,and 81802506) Liaoning Provincial Natural Science Foundation of China(Grant No.201602244) Liaoning Province Innovation Talents Support Program in Colleges and Universities(Grant No.LR2016023) Distinguished Professor Project of Liaoning Province Special Grant for Translational Medicine,Dalian Medical University(Grant No.2015002) Basic Research Projects in Colleges and Universities of Liaoning Province(Grant No.LQ2017033) 

主　　题：SNRPG glioblastoma cell cycle temozolomide therapy 

摘      要：Objective:Temozolomide(TMZ)is commonly used for glioblastoma multiforme(GBM)***,drug resistance limits its therapeutic effect in GBM ***-binding proteins(RBPs)have vital roles in posttranscriptional *** disturbance of RBP-RNA network activity is potentially associated with cancer development,the precise mechanisms are not fully *** SNRPG gene,encoding small nuclear ribonucleoprotein polypeptide G,was recently found to be related to cancer incidence,but its exact function has yet to be ***:SNRPG knockdown was achieved via short hairpin *** expression profiling and Western blot analyses were used to identify potential glioma cell growth signaling pathways affected by *** tumors were examined to determine the carcinogenic effects of SNRPG on glioma ***:The SNRPG-mediated inhibitory effect on glioma cells might be due to the targeted prevention of Myc and *** addition,the effects of SNRPG loss on p53 levels and cell cycle progression were found to be ***,SNRPG was increased in TMZ-resistant GBM cells,and downregulation of SNRPG potentially sensitized resistant cells to TMZ,suggesting that SNRPG deficiency decreases the chemoresistance of GBM cells to TMZ via the p53 signaling *** data confirmed that SNRPG suppression sensitizes GBM cells to TMZ by targeting Myc via the p53 signaling ***:These results indicated that SNRPG is a probable molecular target of GBM and suggested that suppressing SNRPG in resistant GBM cells might be a substantially beneficial method for overcoming essential drug resistance.