Transcriptional Profile Alteration of Peripheral Blood in Chronic Hypoxia

作     者：Tingting Wang Junyue Xing Lijing Zhang Hao Zhang Tingting Wang;Junyue Xing;Lijing Zhang;Hao Zhang

作者机构：State Key Laboratory of Cardiovascular DiseasesFuwai HospitalNational Center for Cardiovascular DiseasesChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100037China Heart Center and Shanghai Institution of Pediatric Congenital Heart DiseasesShanghai Children's Medical CenterNational Children's Medical CenterShanghai Jiao Tong University School of MedicineShanghai 200127China Children's Heart CenterThe Second Affiliated Hospital and Yuying Children's HospitalInstitute of Cardiovascular Development and Translational MedicineWenzhou Medical UniversityWenzhouZhejiang 325027China 

出 版 物：《Chinese Medical Sciences Journal》 (中国医学科学杂志（英文版）)

年 卷 期：2020年第35卷第1期

页      面：54-64页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 

基　　金：the National Science Fund for Distinguished Young Scholars(81525002 2016-2020) 

主　　题：chronic hypoxia RNA-Seq peripheral blood cyanotic congenital heart diseases 

摘      要：Objective Many physiological and pathological conditions,including cyanotic congenital heart diseases(CCHD),are accompanied by chronic hypoxia,which might interfere with the transcription ***,the transcriptome profile in peripheral blood under hypoxia is still *** present work aimed to explore the transcriptional profile alteration of peripheral blood in chronic *** The present study used a chronic hypoxia rat model to simulate the hypoxic state of CCHD *** groups of Sprague-Dawley rats(n=6 per group)were either exposed to hypoxia(10%O2)or normoxia(21%O2)for 3 *** weight was measured *** blood was collected and total RNA was extracted for RNA-Seq at the end of the hypoxia *** quality assessment,the library was sequenced by the Illumina Hiseq *** differentially expressed genes were screened(false discovery rate2).The functional annotation analysis and cluster analysis of differentially expressed genes were performed based on the adjusted P-value(padj0.05).Results Compared with the control group,the body weight of the rats in the hypoxia group was significantly lowered(P0.01).RNA-Seq results showed that the transcriptome patterns of the two groups had significant *** total,872 genes were identified as differentially *** all,803 genes were downregulated,while only 69 genes were up-regulated in the hypoxia *** functional enrichment analysis of the 872 genes showed that multiple biological processes involved,such as porphyrin-containing compound metabolic process,hemoglobin complex and oxygen transporter *** Our study demonstrated the transcriptional profile alteration in peripheral blood of chronic hypoxia rat *** study provided basic data and directions to further understand the physiological and pathological changes in patients with CCHD.