Polysaccharide-based chromatographic adsorbents for virus purification and viral clearance

作     者：Guy-Alain Junter Laurent Lebrun Guy-Alain Junter;Laurent Lebrun

作者机构：Normandie UnivUNIROUENINSA RouenCNRSPBS76000RouenFrance 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2020年第10卷第4期

页      面：291-312页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 0817[工学-化学工程与技术] 100102[医学-免疫学] 0703[理学-化学] 0702[理学-物理学] 10[医学] 

主　　题：Polysaccharide Chromatography Virus particle Biopharmaceuticals purification Vaccine 

摘      要：Viruses still pose a significant threat to human and animal health *** the fight against viral infections,high-purity viral stocks are needed for manufacture of safer *** is also a priority to ensure the viral safety of biopharmaceuticals such as blood *** techniques are widely implemented at both academic and industrial levels in the purification of viral particles,whole viruses and virus-like particles to remove viral contaminants from biopharmaceutical *** paper focuses on polysaccharide adsorbents,particulate resins and membrane adsorbers,used in virus purification/removal chromatography *** chromatographic modes are surveyed,with particular attention to ion exchange and affinity/pseudo-affinity adsorbents among which commercially available agarose-based resins(Sepharose®)and cellulose-based membrane adsorbers(Sartobind®)occupy a dominant *** built on the development of new ligands coupled to conventional agarose/cellulose matrices,the development perspectives of polysaccharide-based chromatography media in this antiviral area are stressed in the conclusive part.