Chimeric Newcastle Disease Virus-like Particles Containing DC-Binding Peptide-Fused Haemagglutinin Protect Chickens from Virulent Newcastle Disease Virus and H9N2 Avian Influenza Virus Challenge

作     者：Xiaohong Xu Jing Qian Lingsong Qin Jindou Li Cong Xue Jiaxin Ding Weiqi Wang Wei Ding Renfu Yin Ningyi Jin Zhuang Ding Xiaohong Xu;Jing Qian;Lingsong Qin;Jindou Li;Cong Xue;Jiaxin Ding;Weiqi Wang;Wei Ding;Renfu Yin;Ningyi Jin;Zhuang Ding

作者机构：Laboratory of Infectious DiseasesCollege of Veterinary MedicineKey Laboratory of Zoonosis ResearchMinistry of EducationJilin UniversityChangchun 130062China College of Basic Medical ScienceJilin UniversityChangchun 130021China Institute of Veterinary MedicineJiangsu Academy of Agricultural Sciences/Key Laboratory for Veterinary Bio-Product EngineeringMinistry of AgricultureNanjing 210014China College of Agriculture and Forestry ScienceLinyi UniversityLinyi 276000China 

出 版 物：《Virologica Sinica》 (中国病毒学（英文版）)

年 卷 期：2020年第35卷第4期

页      面：455-467页

核心收录：

学科分类：0710[理学-生物学] 090603[农学-临床兽医学] 1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 09[农学] 0906[农学-兽医学] 

基　　金：supported by grants from the National Key Research and Development Program of China(Grant No.2018YFD0500100) the National Natural Science Foundation of China(Grant Nos.31772735,31472195) the Jiangsu Provincial Natural Science Foundation of China(Grant No.BK20180299) Jiangsu Agriculture Science and Technology Innovation Fund CX(19)3019 the Key Technology Research and Development Project of Jilin Province(Grant No.20180201021NY) 

主　　题：Newcastle disease virus-like particles DC-binding peptide H9N2 Secretory immunoglobulin A(sIgA) Candidate vaccine 

摘      要：Newcastle disease virus(NDV)and H9N2 subtype Avian influenza virus(AIV)are two notorious avian respiratory pathogens that cause great losses in the poultry *** inactivated commercial vaccines against NDV and AIV have the disadvantages of inadequate mucosal responses,while an attenuated live vaccine bears the risk of *** cell(DC)targeting strategies are attractive for their potent mucosal and adaptive immune-stimulating ability against respiratory *** this study,DC-binding peptide(DCpep)-decorated chimeric virus-like particles(cVLPs),containing NDV haemagglutinin–neuraminidase(HN)and AIV haemagglutinin(HA),were developed as a DC-targeting mucosal vaccine ***-decorated cVLPs activated DCs in vitro,and induced potent immune stimulation in chickens,with enhanced secretory immunoglobulin A(sIgA)secretion and splenic T cell differentiation.40μg cVLPs can provide full protection against the challenge with homologous,heterologous NDV strains,and AIV *** addition,DCpep-decorated cVLPs could induce a better immune response when administered intranasally than intramuscularly,as indicated by robust s IgA secretion and a reduced virus shedding *** together,this chimericVLPs are a promising vaccine candidate to control NDV and AIV H9N2 and a useful platform bearing multivalent antigens.