Protective effects of iptakalim,a novel ATP-sensitive potassium channel opener,on global cerebral ischemia-evoked insult in gerbils
Protective effects of iptakalim,a novel ATP-sensitive potassium channel opener,on global cerebral ischemia-evoked insult in gerbils作者机构：Department of Anatomy Laboratory of Neuropharmacology Histology and Pharmacology Nanjing Medical University Nanjing China Department of Cardiovascular Pharmacology Beijing Institute of Pharmacology and Toxicology Beijing China
出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))
年 卷 期：2006年第27卷第6期
页 面：665-672页
核心收录：
学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学]
基 金：国家自然科学基金 江苏省卫生厅重点项目 江苏省教育厅自然科学基金 国家教育部高等学校博士学科点专项科研基金
主 题：ATP-sensitive potassium channel opener iptakalim global cerebral ischemia neuroprotection excitotoxicity
摘 要：Aim:To investigate the protective role of iptakalim,a novel ATP sensitive potas-sium channel opener,on global cerebral ischemia-evoked insult in gerbils andglutamate-induced PC 12 cell ***:Global cerebral ischemia was in-duced by occluding the bilateral common carotid arteries in gerbils for 5 *** field maze and T-maze were employed to investigate the experimental thera-peutic value of iptakalim on ischemic brain insult（n=8）.The pyramidal cells in thehippocampal CA1 regions were counted to assess the protective effects *** released from the gerbil hippocampus and PC12 cells weredetermined by *** calcium was measured by Fluo-3 AM with ABio-Rad Radiance 2100TM confocal system in conjunction with a Nikon *** glutamate uptake measurements were determined by liq-uid scintillation ***:Iptakalim（0.5-4.0 mg/kg per day,ip）couldreduce the high locomotor activity evoked by ischemia and improve global cere-bral ischemia-induced working memory *** studies revealedthat iptakalim could increase the survival neuron in the hippocampus CA1 zone ina dose-dependent ***,iptakalim could reverse ischemia-evokedincreases of glutamate in the hippocampus of *** an in vitro study,iptakalimprotected PC12 cells against glutamate-induced excitotoxicity,reduced the[Ca2+]iincreases,and enhanced the glutamate uptake activity of primary ***:Iptakalim plays a key role in preventing global cerebralischemia-evoked insults in gerbils and glutamate-induced PC12 cell injury by *** might be a promising novel candidate for the preventionand/or treatment of stroke.
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