Expression of P53 during Lens Epithelial Cell Apoptosis Induced by Ultraviolet

作     者：孙旭芳 邹尉玉 赵长松 SUN XUFANG;ZOU WEIYU;ZHAO CHANGSONG DEPARTMENT OF OPHTHALMOLOGY;TONGJI HOSPITAL;TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITY OF SCIENCE AND TECONOLOGY;WUHAN

作者机构：Department of Ophthalmology Tongji Hospital Tongji Medical College Huazhong University of Science and Teconology Wuhan 430030 

出 版 物：《Journal of Huazhong University of Science and Technology(Medical Sciences)》 (华中科技大学学报（医学英德文版）)

年 卷 期：2001年第21卷第3期

页      面：263-264页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

主　　题：apoptosis lens epithelial cell ultraviolet cataract P53 

摘      要：The apoptosis of lens epithelial cells (LECs) induced by ultraviolet and the expression of P53 were investigated. Wistar rats received 100 mW/m 2 ultraviolet irradiation (UVR) (λ=280 nm-315 nm) for 15 min. One, 6, 24 h after irradiation the lens capsules were dissected. The percentages of apoptotic cells were evaluated by the TdT-dUTP terminal nick-end labeling (TUNEL) technique and the expression of P53 was detected by using immunohistochemical assay. The results showed that the percentages of TUNEL-positive nuclei at 24 h after irradiation was significantly higher than in the control group and those 1 h, 6 h after irradiation. The percentages of P53-positive cells at 6 h, 24 h after irradiation were significantly higher than in the control group and those 1 h after irradiation. It was concluded that UVR could induce the apoptosis of lens epithelial cell. The expression of P53 might be responsible for the apoptosis of lens epithelial cells.