卵巢储备减少妇女增值或凋亡相关标记物的卵巢表达

作     者：Vital-Reyes V. Rodrguez-Burford C. Chhieng D.C. W.E. Grizzle 张旸 

作者机构：Department of Pathology University of Alabama at Birmingham Birmingham AL United States Dr. 

出 版 物：《世界核心医学期刊文摘（妇产科学分册）》 (Core Journal in Obstetrics/Gynecology)

年 卷 期：2006年第2卷第11期

页      面：43-44页

学科分类：1002[医学-临床医学] 100211[医学-妇产科学] 10[医学] 

主　　题：卵巢储备 末端标记 雄激素受体 caspase 子宫切除术 克罗米芬 脱氧核糖核苷 雌激素受体 免疫 

摘      要：Objective: To determine DNA fragmentation and several molecules associated with apoptosis or proliferation in ovaries of patients with diminished ovarian reserve. Design: Cross-sectional analysis. Setting: Tertiary institutional hospital. Patient(s): Patients with benign uterine pathology who had undergone a hysterectomy and oophorectomy were categorized by the citrate clomiphene challenge test in diminished ovarian reserve or control group. Intervention(s): None. Main Outcome Measure(s): Apoptosis was determined using TUNEL (terminal deoxynucleotidyl transferase biotin-dUTP nick end- labeling) assay and p53, p27, Bax, caspase- 3, caspase- 8, caspase- 9, Fas- L, Bcl- 2, GATA- 4, Ki- 67, proliferating- cell nuclear antigen (PCNA), estrogen receptor, P receptor, and androgen receptor expression by immunohistochemistry. Result( s): Fifteen patients were studied. DNA fragmentation and expression of Bax, caspase-3, caspase-8, caspase-9, Fas-L, Bcl- 2, GATA- 4, Ki- 67, and PCNA were observed in the whole ovary in both groups. In the control group, the expression of caspase- 3 and caspase-8 in the ovarian stroma was significantly higher. Conclusion(s): DNA fragmentation and the expression of several molecules that participate in ovarian proliferation or apoptosis are present in cycling ovaries, but these markers were not significantly different in patients with diminished ovarian reserve. Thus, the mechanism leading to diminished ovarian reserve does not involve an easily detectable dysregulation in apoptosis or proliferation of ovarian follicles.