Statistical modeling of nitrogen-dependent modulation of root system architecture in Arabidopsis thaliana

作     者：Takao Araya Takuya Kubo Nicolaus von Wiren Hideki Takahashi 

作者机构：Department of Biochemistry and Molecular BiologyMichigan State UniversityEast LansingM148824USA Molecular Plant NutritionLeibniz Institute of Plant Genetics and Crop Plant ResearchD-06466 GaterslebenGermany Graduate School of Environmental ScienceHokkaido UniversitySapporo 060-0810Japan 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2016年第58卷第3期

页      面：254-265页

核心收录：

学科分类：09[农学] 0903[农学-农业资源与环境] 

基　　金：supported in part by the National Science Foundation(IOS-1444549 to H.T.) the Deutsche Forschungsgemeinschaft(WI1728/13-1 to N.v.W.) Grants-in-aid for Scientific Research from the Ministry of Education,Culture,Sports,Science,and Technology of Japan(T.K.) 

主　　题：Ammonium mathematical modeling nitrate nitrogen root system architecture 

摘      要：Plant root development is strongly affected by nutrient availability. Despite the importance of structure and function of roots in nutrient acquisition,statistical modeling approaches to evaluate dynamic and temporal modulations of root system architecture in response to nutrient availability have remained as widely open and exploratory areas in root biology. In this study,we developed a statistical modeling approach to investigate modulations of root system architecture in response to nitrogen availability. Mathematical models were designed for quantitative assessment of root growth and root branching phenotypes and their dynamic relationships based on hierarchical con figuration of primary and lateral roots formulating the fishbone-shaped root system architecture in Arabidopsis thaliana. Time-series datasets reporting dynamic changes in root developmental traits on different nitrate or ammonium concentrations were generated for statistical analyses. Regression analyses unraveled key parameters associated with：（i） inhibition of primary root growth under nitrogen limitation or on ammonium;（ii） rapid progression of lateral root emergence in response to ammonium; and（iii） inhibition of lateral root elongation in the presence of excess nitrate or ammonium. This study provides a statistical framework for interpreting dynamic modulation of root system architecture,supported by metaanalysis of datasets displaying morphological responses of roots to diverse nitrogen supplies.