Cathepsin C promotes the progression of periapical periodontitis

作     者：Wei Yin Ming Dong Dandan Ye Qicheng Liu Shuo Liu Chun Shi Hua Bai Qian Wang Xue Yang Lina Wang Weidong Niu 尹伟;董明;叶丹丹;刘启成;刘硕;史春;白桦;王倩;杨雪;王丽娜;牛卫东

作者机构：The State Key Laboratory Breeding Base of Basic Science of Stomatology(Hubei-MOST)&Key Laboratory of Oral Biomedicine(Ministry of Education)School&Hospital of StomatologyWuhan UniversityWuhan 430079China Department of Biomedical Data ScienceGeisel School of Medicine at Dartmouth CollegeOne Medical Center DriveLebanonNH 03756USA School of StomatologyDalian Medical UniversityDalian 116044China Hospital of StomatologyYiwu CityJinhua 322005China 

出 版 物：《Science Bulletin》 (科学通报（英文版）)

年 卷 期：2020年第65卷第11期

页      面：951-957,M0004页

核心收录：

学科分类：1003[医学-口腔医学] 100302[医学-口腔临床医学] 10[医学] 

基　　金：This work was supported by the Open Research Fund Program of State Key Laboratory of Oral Disease Sichuan Univeristy China(SKLOD2019OF06) 

主　　题：Cathepsin C Receptor activator of nuclear factor-B ligand Periapical periodontitis Tartrate resistant acid phosphatase 

摘      要：Although the role of cathepsin C (Cat C) in inflammation is gradually being elucidated, its function in periapical periodontitis, which is one of the most common infectious diseases worldwide, has not been studied. This study evaluated a surgically-induced model of periapical periodontitis in cathepsin C (Cat C) knock-down (KD) mice, which was constructed with a tetracycline operator, to evaluate the role of Cat C in the pathogenesis and progression of periapical periodontitis. Our results showed, for the first time, that there was a statistically significant increase in the expression of Cat C as periapical periodontitis progressed;this increase started from 1 week after surgery and reached a peak at 3 weeks after surgery, before gradually decreasing. The volume of periapical bone resorption in Cat C KD mice was significantly smaller than that in wild-type mice at 3 and 4 weeks after surgery (P0.05). Inflammatory cell infiltration into the apical tissues of wild-type mice was also significantly higher than that of Cat C KD mice. The expression of receptor activator of nuclear factor-j B ligand (RANKL) in wild-type mice was also higher than that in Cat C KD mice. The difference in the number of osteoclasts in the apical area between the two groups was statistically significant after 2 weeks. Correlation analysis showed that there was a significant correlation between Cat C and RANKL expression (r= 0.835). Therefore, our data indicated that Cat C promoted the apical inflammation and bone destruction in mice.