Base editing in plants: Current status and challenges

作     者：Sutar Suhas Bharat Shaoya Li Jingying Li Lei Yan Lanqin Xia Sutar Suhas Bharat;Shaoya Li;Jingying Li;Lei Yan;Lanqin Xia

作者机构：Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2020年第8卷第3期

页      面：384-395页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：partly funded by the Transgenesis Initiative Project supported by the Ministry of Agriculture and Rural Affairs of China(2019ZX08010001,2019ZX08010003) the Central Non-Profit Fundamental Research Funding supported by the Institute of Crop Sciences,Chinese Academy of Agricultural Sciences(S2018QY05) 

主　　题：Base editing in plants Current status and challenges 

摘      要：Genome editing technologies have revolutionized the field of plant science by enabling targeted modification of plant genomes and are emerging as powerful tools for both plant gene functional analyses and crop improvement. Although homology-directed repair(HDR)is a feasible approach to achieve precise gene replacement and base substitution in some plant species, the dominance of the non-homologous end joining pathway and low efficiency of HDR in plant cells have limited its application. Base editing has emerged as an alternative tool to HDR-mediated replacement, facilitating precise editing of plant genome by converting one single base to another in a programmable manner without a doublestranded break and a donor repair template. In this review, we summarize the latest developments in base-editing technologies as well as their underlying mechanisms. We review current applications of these technologies in plant species. Finally, we address the challenges and future perspectives of this emerging technology in plants.