Indole alkaloid glycosides with a 1′-(phenyl)ethyl unit from Isatis indigotica leaves

作     者：Qinglan Guo Dawei Li Chengbo Xu Chenggen Zhu Ying Guo Haibo Yu Xiaoliang Wang Jiangong Shi Qinglan Guo;Dawei Li;Chengbo Xu;Chenggen Zhu;Ying Guo;Haibo Yu;Xiaoliang Wang;Jiangong Shi

作者机构：State Key Laboratory of Bioactive Substance and Function of Natural MedicinesInstitute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050China 

出 版 物：《Acta Pharmaceutica Sinica B》 (药学学报（英文版）)

年 卷 期：2020年第10卷第5期

页      面：895-902页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Financial support of the National Natural Science Foundation of China(81630094,21732008,and 81730093) CAMS Innovation Fund for Medical Science of China(2017-I2M-3-010 and 2016-I2M1-010) the Drug Innovation Major Project(2018ZX09711001001-001,China) 

主　　题：Cruciferae Isatis indigotica Indole alkaloid glycoside Isatidifoliumosides Epiisatidifoliumosides KCNQ2 inhibition Anti-infuenza Anti-virus Activity 

摘      要：Seven indole alkaloid glycosides containing a 1′-(4″-hydroxy-3″,5″-dimethoxyphenyl)ethyl unit(1-7)were isolated from an aqueous extract of Isatis indigotica leaves(da qing ye).Their structures were determined by spectroscopic data analysis combined with enzymatic hydrolysis as well as comparison of their experimental CD(circular dichroism)and calculated ECD(electrostatic circular dichroism)*** on analysis of[α]D20 and/or Cotton effect(CE)data of 1-7,two simple roles to assign location and/or configuration ofβ-glycopyranosyloxy and 1′-(phenyl)ethyl units in the indole alkaloid glycosides are *** in plausible biosynthetic pathways of 1-7 is *** 3 and 4 and their mixture in a 3:2 ratio showed activity against KCNQ2 in CHO *** mixture of 5 and 6(3:2)exhibited antiviral activity against influenza virus H1 N1 PR8 with IC5064.7μmol/L(ribavirin,IC5054.3μmol/L),however,the individual 5 or 6 was *** structure-activity relationships were observed.