Simultaneous Determination of Amoxicillin, Lansoprazole, and Levofloxacin in Pharmaceuticals by HPLC with UV-Vis Detector

作     者：Mustafa Gülfen YazgiCanbaz AbdilÖzdemir Mustafa Gülfen;Yazgi Canbaz;Abdil ?zdemir

作者机构：Department of ChemistryFaculty of Arts and SciencesSakarya University54187 SakaryaTurkey 

出 版 物：《Journal of Analysis and Testing》 (分析检测（英文）)

年 卷 期：2020年第4卷第1期

页      面：45-53页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0703[理学-化学] 

主　　题：HPLC Amoxicillin Levofloxacin Lansoprazole Simultaneous determination 

摘      要：In this study,a specific and rapid high-performance liquid chromatography(HPLC)method has been developed and validated for the simultaneous determination of amoxicillin,lansoprazole,and levofloxacin in *** was used as internal standard(IS)in the ***-Vis absorption spectra of the analytes and the IS were taken for the determination of suitable absorption wavelength of UV-Vis detector(diode array detector,DAD)in the HPLC instrument.A reverse-phase C18 column was used in the separation and determination of amoxicillin,lansoprazole,and levofloxacin together with the *** pharmaceutical analytes were quantified by the UV-Vis diode array detector in the HPLC using MeOH-0.01 M CH_(3)COONH_(4)(70:30)as the mobile *** linear calibration curves of them were measured in the ranges of 15-40 mg/L,2.5-15.0 mg/L,and 7.5-20.0 mg/L for amoxicillin,lansoprazole,and levofloxacin,*** calibration correlations(R^(2):0.9942,0.9997,and 0.9974)were *** percentage recoveries of the amoxicillin,lan-soprazole,and levofloxacin in commercial pharmaceuticals were obtained as 105.5%,98.57%,and 102.5%,*** results showed that amoxicillin,lansoprazole,and levofloxacin together with paracetamol IS could be separated and determined simultaneously with low LOD and LOQ values using the proposed HPLC method.