Circ-Smad5 retards the G1/S transition of cell cycle via inhibiting the activity of wnt/lef/cyclind1 signaling in JB6 cells

作     者：Xiaogen Ma Fei Xiang Zhuo Pei Jiafeng Miao Pan Wu Xiaofeng Song Yuhong Li Yiming Zhang 

作者机构：Department of Plastic and Cosmetic SurgeryXinqiao HospitalArmy Medical UniversityChongqing400037PR China Department of Cell BiologyArmy Medical UniversityChongqing400038PR China Institute of Burn ResearchState Key Laboratory of TraumaBurns and Combined InjurySouthwest HospitalArmy Medical UniversityChongqing400038PR China Department of Biomedical EngineeringNanjing University of Aeronautics and AstronauticsNanjing211106PR China 

出 版 物：《Genes & Diseases》 (基因与疾病（英文）)

年 卷 期：2021年第8卷第3期

页      面：364-372页

核心收录：

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100101[医学-人体解剖与组织胚胎学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China[grant number 81472895] the Natural Science Foundation of Chongqing[grant number cstc2018jcyjAX0053] Key Talents Support Plan of Army Medical University(2019).We thank Min Gao and Yizhan Xing at the Army Medical University for technical support 

主　　题：Cell cycle Circ-Smad5 Circular RNA Wnt signaling Cell proliferation 

摘      要：Circular RNAs are a large class of noncoding ***5 functions in cell differentiation,cell proliferation and *** has been reported that lnc-Smad5 can inhibit the proliferation of diffuse large B cell ***,the function of circ-Smad5 has not yet been *** vectors were constructed to establish circ-Smad5 upregulated and circ-Smad5 downregulated cell models.A CCK-8 assay was used to detect the proliferation of JB6 *** was used to analyze the cell cycle in the cell *** blot,immunofluorescence staining and TOP/FOP flash dual luciferase activity assays were used to determine the activity of the Wnt signaling *** results revealed that the expression level of circ-Smad5 in JB6 cells was significantly lower than the expression level of *** with the control group,the percentage of S phase cells and the expression level of cyclin D1 protein were significantly higher in the sh-circ-Smad5 *** the sh-circSmad5 group,b-catenin and LEF-1 were significantly increased,p-b-catenin was significantly decreased,and the relative activity of the TOP/FOP reporter gene was higher compared to the control group *** phenomena could be reversed by treating with Wnt signaling inhibitor *** conclude that the circ-Smad5 retards the proliferation and the cell cycle progression of JB6 ***,circ-Smad5 may function by inhibiting the activation of Wnt/b-catenin/Lef 1 signaling,which inhibits the expression of cyclin *** the best of our knowledge,we are the first to report the function of circ-Smad5.