The protective effect of caffeine on DNA photosensitive damage:A gel electrophoresis

作     者：HUANG Liping MA Jianhua 

作者机构：College of Biology Engineering Jimei University Xiamen 361021 China 

出 版 物：《Nuclear Science and Techniques》 (核技术（英文）)

年 卷 期：2009年第20卷第4期

页      面：223-227页

核心收录：

学科分类：07[理学] 070202[理学-粒子物理与原子核物理] 0702[理学-物理学] 

基　　金：Supported by Natural Science Foundation of Fujian Province,China (2009J01032) Education Committee of Fujian Province,China (JA081336) Specialized Foundation for Creative Team of Young and Middle-aged Teachers of Jimei University (2006A003) 

主　　题：凝胶体 电泳 DNA UV照射 核技术 

摘      要：Agarose gel electrophoresis was performed to study interaction effect of caffeine on photosensitive injury of DNA caused by anthraquinone-2-sulphonic acid disodium(AQS),a model compound of strong photosensitizer,under 254 nm or 365nm UV irradiation Photosensitive injury of DNA induced by AQS under deoxidized condition was used as *** results show that caffeine may resist effectively the injury effect of photosensitive damage and strong UV irradiation on *** effects depend on the caffeine and AQS concentration,and irradiation *** in concentration of 0.01-3.0μg/μL,may prevent DNA from damage induced by UV light,but caffeine in concentration of5.0μg/μL accelerates the DNA *** particular,in the aqueous solution system of DNA,caffeine and AQS,at pH 6.25-7.35,the caffeine in concentration of 2.5-4.50μg/μL may resist the photosensitive injury of DNA caused by AQS under the deoxidized condition and exposure by 254 nm UV for 10 *** caffeine in concentration of 5μg/μL would present a synergetic effect on the photosensitive injury of *** molecular mechanism also is discussed.