Inhibition of the negative effect of high glucose on osteogenic differentiation of bone marrow stromal cells by silicon ions from calcium silicate bioceramics

作     者：Xixi Dong Xiaoya Wang Min Xing Cancan Zhao Bin Guo Junkai Cao Jiang Chang 

作者机构：Stomatology DepartmentGeneral Hospital of Chinese PLA28 Fu Xing RoadBeijing 100853PR China State Key Laboratory of High Performance Ceramics and Superfine MicrostructureShanghai Institute of CeramicsChinese Academy of Sciences1295 Dingxi RoadShanghai 200050PR China 

出 版 物：《Regenerative Biomaterials》 (再生生物材料（英文版）)

年 卷 期：2020年第7卷第1期

页      面：9-17页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0836[工学-生物工程] 

基　　金：This work was supported by the Natural Science Foundation of China[Grant No.31271054 and 31770980] 

主　　题：high glucose silicate osteogenic differentiation bone tissue engineering 

摘      要：Human bone marrow stem cells(hBMSCs)are exploited for miscellaneous applications in bone tissue engineering where they are mainly used as seed ***,high glucose(HG)environment has negative impacts on the proliferation and osteogenic differentiation of hBMSCs,thus reducing the bone formation in diabetic *** our former research works,we discovered that silicon(Si)ions extracted from silicate-based bioceramics are able to stimulate the proliferation and osteogenic differentiation of hBMSCs under normal culture *** study aimed to investigate if Si ions could prevent HG-induced inhibition of proliferation and osteogenesis of *** found that 2.59ppm concentration of Si ions promoted the proliferation of hBMSCs under HG *** results from alkaline phosphatase(ALP)activity assay,Alizarin red S staining and quantitative real-time PCR analysis of osteogenic genes(BMP2,RUNX2,ALP,COL1 and OCN)demonstrated that the 15.92ppm concentration of Si ions prevented HG-induced inhibition of the osteogenic differentiation of ***,application of Si ions reduced the level of reactive oxygen species in HG-treated *** HG-treated hBMSCs following 15.92ppm Si ions treatment,activation of BMP2/SMAD signaling pathway was detected,as indicated by the increased expression of BMP2 receptors and its downstream genes such as SMAD1,SMAD4 and *** together,we provide evidence that the specific concentration of Si ions compensated HG-induced inhibition of proliferation and osteogenic differentiation of hBMSCs through antioxidant effect and modulation of BMP2/SMAD *** results suggest that silicate-based bioceramics might be good scaffold biomaterials for bone engineering applications in diabetes patients.