Identification of sumoylated targets in proliferating mouse spermatogonia and human testicular seminomas

作     者：Margarita Vigodner Benjamin Lucas Stav Kemeny Tamar Schwartz Rebecca Levy Margarita Vigodner;Benjamin Lucas;Stav Kemeny;Tamar Schwartz;Rebecca Levy

作者机构：Department of BiologyStern CollegeYeshiva UniversityNew YorkNY 10016USA Department of Developmental and Molecular BiologyAlbert Einstein College of MedicineBronxNY 10461USA 

出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)

年 卷 期：2020年第22卷第6期

页      面：569-577页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Selma and Jacques H. Mitrani Foundation National Institutes of Health, NIH National Institute of Child Health and Human Development, NICHD, (R15HD067944) Yeshiva University, YU 

主　　题：proliferation seminoma spermatog onia sumoylation 

摘      要：Spermatogenesis is regulated by a complex network of posttranslation ***(a modification by small ubiquiti like modifiers,or SUMO proteins)was identified as an importa nt cellular event in d iff ere nt cell *** proteins are highly expressed in the testis,and their role during spermatogenesis has begun to be *** the important role of sumoylation in the regulation of mitosis and cancer progress!on in other tissues,the aim of the current study was to identify the targets of SUMO in proliferating mouse spermatog onia and huma n semi noma tissues and to in itially exami ne the level of sumoylation in relation to the proliferative activity of the *** freshly purified spermatogonia and Cl8-4 spermatogonia cell line,mass spectrometry analysis identified several SUMO targets implicated into the proliferation of spermatogonia(such as heat shock protein 60[HSP60]and prohibitin).Tissue array and western blot approaches showed that SUMO expression is a prominent feature of human seminomas and that the proliferative activity of the tumor tissues was positively correlated with the level of SUMO *** regulation of sumoylati on with si-RNA was not sufficie nt to sign ificantly affect the proliferation of C18-4 spermatogonia;however,SUMO overexpression in creased the proliferation rate of the *** data suggest that cells are more sensitive to an elevated level of SUMO,and that this situation may lead to an upregulated cellular proliferation and,possibly,*** spectrometry analysis identified around a hundred SUMO targets in seminoma ***,many of the identified proteins(such as proliferating cell nuclear antigen[PCNA],DNA topoisomerase 2-alpha[Top2A],prohibitin,14-3-3 protein,and others)were implicated in oncogenic transformation and cancer progression.