Comparison of ART outcomes in men with altered mRNA protamine 1/protamine 2 ratio undergoing intracytoplasmic sperm injection with ejaculated and testicular spermatozoa
作者机构：iGLSAlicante 03540SpainMolecular AndrologyJustus-Liebig-UDiversity GiessenGiessen 35385Germany IVF-SpainAlicante 03540SpainMolecular AndrologyJustus-Liebig-UDiversity GiessenGiessen 35385Germany Department of UrologyPediatric Urology and AndrologyMolecular AndrologyJustus-Liebig-UDiversity GiessenGiessen 35385Germany
出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)
年 卷 期：2020年第22卷第6期
页 面：623-628页
核心收录：
学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学]
基 金：The authors thank all the patients for consenting to participate in this study.Furthermore the technical assistance of Barbara Frohlich and Mareike Buch-Heberling is gratefully acknowledged.KS was supported by a Research Grant from the University Medical Center Giessen and Marburg(UKGM project 29/2015GI).This research did not receive any other specific grant from funding agencies in the public commercial or not-for-profit sectors
主 题：DNA damage male infertility mRNA protamine ratio sperm chromatin testicular spermatozoa
摘 要：Assisted reproductive technologies invoIving the use of spermatozoa and eggs for in vitro fertilization(IVF)have come as the solution for many infertile couples to become ***,in some cases,the use of ejaculated spermatozoa delivers poor IVF *** studies have suggested the use of testicular spermatozoa in severe male in fertility cases,but no guideli nes regarding their utilization are currently *** the present study,we found the mRNA protamine 1/protamine 2(P1/P2)ratio to be a valuable biomarker of poor sperm function that could be used as a diagnostic key for the identification of cases that would benefit from the use of testicular spermatozoa.A total of 23 couples undergoing egg donation cycles with at least one previous cycle failure were *** couples underwent two consecutive intracytoplasmic sperm injection(ICSI)cycles with either ejaculated or testicular spermatozoa(TESA).The sperm mRNA P1/P2 ratio,fertilization rate,blastocyst rate,and pregnancy and live birth rate were *** showed improved ICSI and clinical outcomes in cycles with testicular spermatozoa in men with altered mRNA P1/P2 *** cycles presented significantly higher rates of fertilization(mean±standard deviation:76.1%±15.1%vs 65.5%±18.8%),blastocyst formation(55.0%±20.3%vs 30.8%±23.8%),and good morphological quality blastocyst(28.9%±22.9%vs 13.5%±17.9%)and also improvements on pregnancy(60.9%vs 0%)and healthy birth rates(56.5%vs 0%)than EJACULATE *** results described here suggest that in patients with previous IVF/ICSI failures and aberrant mRNA protamine ratios,the use of testicular spermatozoa may be a good alternative to improve clinical outcomes.
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