Changes in extracellular matrix in different stages of colorectal cancer and their effects on proliferation of cancer cells

作     者：Zhu-Lin Li Zhen-Jun Wang Guang-Hui Wei Yong Yang Xiao-Wan Wang 

作者机构：Department of General SurgeryBeijing Chaoyang HospitalCapital Medical UniversityBeijing 100020China Institute of Basic Medical SciencesChinese Academy of Medical SciencesBeijing 100005China 

出 版 物：《World Journal of Gastrointestinal Oncology》 (世界胃肠肿瘤学杂志（英文版）（电子版）)

年 卷 期：2020年第12卷第3期

页      面：267-275页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Colorectal cancer Extracellular matrix MMP Proliferation Collagen TIMP 

摘      要：BACKGROUND The extracellular matrix is the main component of the tumor *** matrix remodels with the oncogenesis and development of *** studies usually focused on the changes of proteins in normal colorectal tissues and colorectal *** is known about the changes in the extracellular matrix in different stages of colorectal cancer and the effects of these changes on the development of this *** To test the changes of type I collagen,type IV collagen,matrix metalloproteinase-2(MMP-2),matrix metalloproteinase-9(MMP-9),and tissue inhibitor of metalloproteinase-3(TIMP-3)in different stages of colorectal cancer and the effects of these changes on the proliferation of cancer *** The extracellular matrix from various stages of colorectal cancer and normal colon tissue was obtained by using acellular *** used proteomics to detect the differential expression of proteins between normal colon tissues and colorectal cancer tissues,and then we used Western blot to observe their expression in each stage of colorectal cancer and in normal colon *** coculturing the extracellular matrix and HT29 colon cancer cells in vivo and in vitro,we tested the cancer cell proliferation rate in vitro by methyl thiazolyl tetrazolium(MTT)assay and in vivo by measuring the tumor *** The expression of type I collagen and MMP-2 increased with increased tumor *** expression of MMP-9 was higher in colorectal cancer tissues and was highest in stage III *** expression of type IV collagen and TIMP-3 decreased with increased tumor *** proliferation rate of cancer cells in the extracellular matrix of colorectal cancer was higher than that in the extracellular matrix of the normal *** These data suggest that the extracellular matrix structure and composition become disorganized during the development of tumors,which is more conducive for the growth of cancer cells.