Comparison between the therapeutic effects of differentiated and undifferentiated Wharton’s jelly mesenchymal stem cells in rats with streptozotocin-induced diabetes

作     者：Chen-Yuan Hsiao Tien-Hua Chen Ben-Shian Huang Po-Han Chen Cheng-Hsi Su Jia-Fwu Shyu Pei-Jiun Tsai 

作者机构：Graduate Institute of Medical SciencesNational Defense Medical CenterTaipei 114Taiwan Department of SurgeryLandseed International HospitalTaoyuan 324Taiwan Institute of Anatomy and Cell BiologySchool of MedicineNational Yang Ming UniversityTaipei 112Taiwan Trauma CenterDepartment of SurgeryVeterans General HospitalTaipei 112Taiwan Division of General SurgeryDepartment of SurgeryVeterans General HospitalTaipei 112Taiwan Department of Obstetrics and GynecologyVeterans General HospitalTaipei 112Taiwan Department of SurgeryCheng Hsin General HospitalTaipei 112Taiwan Department of Biology and AnatomyNational Defense Medical CenterTaipei 114Taiwan Department of Critical Care MedicineVeterans General HospitalTaipei 112Taiwan 

出 版 物：《World Journal of Stem Cells》 (世界干细胞杂志（英文版）（电子版）)

年 卷 期：2020年第12卷第2期

页      面：139-151页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Taipei Veterans General Hospital,No.V106B-024 Yen Tjing Ling Medical Foundation,No.CI-106-20 Cheng Hsin General Hospital,No.CY10716 Taiwan Ministry of Science and Technology,No.MOST 105-2314-B-010-010-MY3 and No.MOST 106-2314-B-010-009 

主　　题：Human Wharton’s jelly mesenchymal stem cell Insulin-producing cells Diabetes mellitus Differentiation Regeneration therapy Anti-inflammatory 

摘      要：BACKGROUND Despite the availability of current therapies,including oral antidiabetic drugs and insulin,for controlling the symptoms caused by high blood glucose,it is difficult to cure diabetes mellitus,especially type 1 diabetes *** Cell therapies using mesenchymal stem cells(MSCs)may be a promising ***,the therapeutic mechanisms by which MSCs exert their effects,such as whether they can differentiate into insulin-producing cells (IPCs) beforetransplantation, are *** this study, we used three types of differentiation media over 10 d to generateIPCs from human Wharton’s jelly MSCs (hWJ-MSCs). We further transplantedthe undifferentiated hWJ-MSCs and differentiated IPCs derived from them intothe portal vein of rats with streptozotocin-induced diabetes, and recorded thephysiological and pathological *** fluorescent staining and C-peptide enzyme-linked immunoassay, we wereable to successfully induce the differentiation of hWJ-MSCs into *** of both IPCs derived from hWJ-MSCs and undifferentiated hWJMSCshad the therapeutic effect of ameliorating blood glucose levels andimproving intraperitoneal glucose tolerance tests. The transplanted IPCs homedto the pancreas and functionally survived for at least 8 wk after transplantation,whereas the undifferentiated hWJ-MSCs were able to improve the insulitis andameliorate the serum inflammatory cytokine in streptozotocin-induced *** IPCs can significantly improve blood glucose levels in diabetic ratsdue to the continuous secretion of insulin by transplanted cells that survive in theislets of diabetic rats. Transplantation of undifferentiated hWJ-MSCs cansignificantly improve insulitis and re-balance the inflammatory condition indiabetic rats with only a slight improvement in blood glucose levels.