Collagen scaffold combined with human umbilical cord-mesenchymal stem cells transplantation for acute complete spinal cord injury

作     者：Wu-Sheng Deng Ke Ma Bing Liang Xiao-Yin Liu Hui-You Xu Jian Zhang Heng-Yuan Shi Hong-Tao Sun Xu-Yi Chen Sai Zhang Wu-Sheng Deng;Ke Ma;Bing Liang;Xiao-Yin Liu;Hui-You Xu;Jian Zhang;Heng-Yuan Shi;Hong-Tao Sun;Xu-Yi Chen;Sai Zhang

作者机构：College of Integrated Traditional Chinese and Western MedicineGansu University of Chinese MedicineLanzhouGansu ProvinceChina Tianjin Key Laboratory of Neurotrauma RepairPingjin Hospital Brain Centercharacteristic medical center of Chinese people’s armed police forceTianjinChina Clinical School of MedicineTianjin Medical UniversityTianjinChina Clinical School of MedicineLogistics University of People’s Armed Police ForceTianjinChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2020年第15卷第9期

页      面：1686-1700页

核心收录：

学科分类：1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 1010[医学-医学技术（可授医学、理学学位）] 100104[医学-病理学与病理生理学] 100215[医学-康复医学与理疗学] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China,Nos.11932013(to SZ),11672332(to SZ) the National Key Research and Development Plan of China,No.2016YFC1101500(to SZ) the Science and Technology Military-Civilian Integration Project of Tianjin of China,No.18ZXJMTG00260(to XYC) the Key Project of Science and Technology Support Plan of Tianjin of China,No.17YFZCSY00620(to XYC) the Rescue Medical Clinical Center Fund of Tianjin of China,No.15ZXLCSY00040(to XYC) 

主　　题：canine collagen scaffolds human human umbilical cord-mesenchymal stem cells nerve regeneration rat spinal cord injury 

摘      要：Currently, there is no effective strategy to promote functional recovery after a spinal cord injury. Collagen scaffolds can not only provide support and guidance for axonal regeneration, but can also serve as a bridge for nerve regeneration at the injury site. They can additionally be used as carriers to retain mesenchymal stem cells at the injury site to enhance their effectiveness. Hence, we hypothesized that transplanting human umbilical cord-mesenchymal stem cells on collagen scaffolds would enhance healing following acute complete spinal cord injury. Here, we test this hypothesis through animal studies and a phase I clinical trial.(1) Animal experiments: Models of completely transected spinal cord injury were established in rats and canines by microsurgery. Mesenchymal stem cells derived from neonatal umbilical cord tissue were adsorbed onto collagen scaffolds and surgically implanted at the injury site in rats and canines;the animals were observed after 1 week–6 months. The transplantation resulted in increased motor scores, enhanced amplitude and shortened latency of the motor evoked potential, and reduced injury area as measured by magnetic resonance imaging.(2) Phase I clinical trial: Forty patients with acute complete cervical injuries were enrolled at the Characteristic Medical Center of Chinese People s Armed Police Force and divided into two groups. The treatment group(n = 20) received collagen scaffolds loaded with mesenchymal stem cells derived from neonatal umbilical cordtissues;the control group(n = 20) did not receive the stem-cell loaded collagen implant. All patients were followed for 12 months. In the treatment group, the American Spinal Injury Association scores and activities of daily life scores were increased, bowel and urinary functions were recovered, and residual urine volume was reduced compared with the pre-treatment baseline. Furthermore, magnetic resonance imaging showed that new nerve fiber connections were formed, and diffusion tens