Suppression of gastric cancer growth by adenovirus-mediated transfer of the PTEN gene

作     者：YingHang Yong-ChenZheng YanCao Qing-ShanLi Yu-JieSui 

作者机构：CentralLaboratoryTheSecondHospitalofJilinUniversityChuangchun130041JilinProvinceChina DepartmentofBiochemistryandMolecularBiologyLifeScienceSchoolofJilinUniversityChuangchun130021JilinProvinceChina 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2005年第11卷第15期

页      面：2224-2229页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Supported by the National Natural Science Foundation of China No. 39970229 

主　　题：PTEN Gastric cancer 

摘      要：AIM: To investigate the tumor-suppressive effect of the phosphatase and tensin homologue deleted from chromosome (PTEN) in human gastric cancer cells th atwere wild type for ***: Adenoviruses expressing PTEN or luciferase as a control were introduced into gastric cancer *** effect of exogenous PTEN gene on the growth and apoptosis of gastric cancer cells that are wtPTEN were examined in vitro and in ***: Adenovirus-mediated transfer of PTEN (AdPTEN) suppressed cell growth and induced apoptosis significantly in gastric cancer cells (MGC-803, SGC-7901)carrying wtPTEN in comparison with that in normal gastric epithelial cells (GES-1) carrying wtPTEN. This suppression was induced through downregulation of the Akt/PKB pathway, dephosphorylation of focal adhesion kinase and mitogen-activated protein kinase and cell-cycle arrest at the G2/M phase but not at the G1 phase. Furthermore,treatment of human gastric tumor xenografts (MGC-803,SGC-7901) with Ad-PTEN resulted in a significant (P0.01)suppression of tumor ***: These results indicate a significant tumorsuppressive effect of Ad-PTEN against human gastric cancer cells. Thus, Ad-PTEN may be used as a potential therapeutic strategy for treatment of gastric cancers.