Subcellular chemical imaging of structurally similar acridine drugs by near-field laser desorption/laser postionization mass spectrometry

作     者：Xiaoling Cheng Zhibin Yin Liu Rong Wei Hang Xiaoling Cheng;Zhibin Yin;Liu Rong;Wei Hang

作者机构：Department of Chemistry and the MOE Key Lab of Spectrochemical Analysis&InstrumentationCollege of Chemistry and Chemical EngineeringXiamen UniversityXiamen 361005China State Key Laboratory of Marine Environmental ScienceXiamen UniversityXiamen 361005China 

出 版 物：《Nano Research》 (纳米研究（英文版）)

年 卷 期：2020年第13卷第3期

页      面：745-751页

核心收录：

学科分类：0808[工学-电气工程] 080901[工学-物理电子学] 0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 080401[工学-精密仪器及机械] 0804[工学-仪器科学与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 0803[工学-光学工程] 0702[理学-物理学] 

基　　金：The authors are grateful for the national support received from the National Natural Science Foundation of China(Nos.21974116 and 21427813) 

主　　题：near-field mass spectrometry acridine drugs single cell imaging laser postionization 

摘      要：Insights into the pharmacologic effect on cellular processes and the potential toxicological effects are vital to new drug development and evaluation,yet research on these subjects remains a great challenge due to the lack of information regarding the spatiotemporal distribution of drugs and metabolites within a single *** spectrometry imaging(MSI)has proven to be a label-free and high-throughput approach for visualizing drug distribution in spatial and temporal ***,single-cell drug imaging has been limited so far by detection sensitivity and microscale lateral ***,we report near-field laser desorption/laser postionization mass spectrometry(NDPI-MS)for single-cell imaging of two structurally similar drugs,proflavine and ethacridine,and subcellular distributions of proflavine at different drug concentrations were *** NDPI-MS imaging results indicate that proflavine was accumulated in lysosomes,which was verified by laser scanning confocal microscopy(LSCM).Additionally,a distinguished subcellular distribution pattern of ethacridine from proflavine could be visualized,highlighting the complexity of the interaction between the drugs and biological environment even though these two drugs possess similar *** together,the present results demonstrate the great potential of the integrated single-cell MSI platform for characterizing the drug distribution and its phenotype changes within individual cells,expediting the identification and evaluation of newly developed drugs.