Effect of Quercetin on Atherosclerosis Based on Expressions of ABCA1, LXR-α and PCSK9 in ApoE-/- Mice

作     者：LI Shan-shan CAO Hui SHEN Ding-zhu CHEN Chuan XING San-li DOU Fang-fang JIA Qing-ling LI Shan-shan;CAO Hui;SHEN Ding-zhu;CHEN Chuan;XlNG San-li;DOU Fang-fang;JIA Qing-ling

作者机构：Shanghai Geriatric Institute of Chinese Medicine Shanghai University of Traditional Chinese Medicine 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2020年第26卷第2期

页      面：114-121页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 10[医学] 100602[医学-中西医结合临床] 

基　　金：Supported by the National Natural Science Foundation of China(No.81202731,81873348) the Shanghai Nature Science Fund(No.16ZR1433900) the Shanghai Health and Family Planning Commission Fund(No.201640217) Shanghai University of Traditional Chinese Medicine Graduate "Innovation Ability Training" Special Research Projects(No.Y201858) 

主　　题：quercetin atherosclerosis ATP binding cassette transporter A1 liver X receptor proprotein convertase subtilisin/kexin type 9 

摘      要：Objective:To investigate the effect of quercetin on ATP binding cassette transporter A1(ABCA1),liver X receptor(LXR),and proprotein convertase subtilisin/kexin type 9(PCSK9)expressions in apoE-knockout(ApoE-/-)mice.Methods:The high-fat diet-induced atherosclerosis(AS)in ApoE-/-mice was established.Thirtysix mice were divided into 3 groups using random number table method:model group(n=12),quercetin group(n=12),and atorvastatin group(n=12),with C57BL/6J mice of the same strain and age as the control group(n=12).Quercetin group and atorvastatin group were administrated with quercetin and atorvastatin by oral gavage,with doses of 12.5 and 4 mg/(kg・d),respectively.Animals in the control and model groups were given an equal volume of distilled water by oral gavage once per day for a total of 12 weeks.Western blot and immunohistochemical methods were employed to determine the aortic ABCA1,LXR-a and PCSK9 protein expressi on.En zyme linked imm uno sorbent assay method was used to detect the expressi on of serum total cholesterol(TC),triglyceride(TG),high density lipoprotein-cholesterol(HDL-C),low density lipoproteincholesterol(LDL-C),tumor necrosis factor-a(TNF-a),interleukin-6(IL-6),and IL-10,combined with tissue pathological exami nation.Results:ApoE-/-mice fed with a high-fat diet had no table atherosclerosis lesions,with reduced ABCA1,LXR-a and IL-10 levels(all P0.01),elevated PCSK9,TNF-a and IL-6 expression,and increased TC and LDL-C con tents(all P0.01).After querceti n in terventi on,the areas of AS plaques and the expressions of PCSK9,TNF-a and IL-6 were significantly reduced(all P0.01),while the expressions of ABCA1 and LXR-a were increased significantly(all P0.01).Conclusion:Quercetin effectively interfered with AS development by regulating the expressions of ABCA1,LXR-a and PCSK9 in ApoE,mice.