Baicalin Reduces Early Brain Injury after Subarachnoid Hemorrhage in Rats

作     者：ZHANG Hua-bin TU Xian-kun SONG Shi-wei LIANG Ri-sheng SHI Song-sheng ZHANG Hua-bin;TU Xian-kun;SONG Shi-wei;LIANG Ri-sheng;SHI Song-sheng

作者机构：Department of NeurosurgeryFujian Medical University Union HospitalNeurosurgery Research Institute of Fujian ProvinceFuzhou(350001)China 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2020年第26卷第7期

页      面：510-518页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by the Project of Medical Innovation of Health and Family Planning Commision of Fujian Province of China (No. 2016-CX-18) the Natural Science Foundation of Fujian Province of China (No. 2017J01204) the Key Young Talents Cultivation Project of Health and Family Planning Commision of Fujian Province of China (No. 2016-ZQN-28) the Excellent Youth Scientific Research Personnel Cultivation Program of Fujian Province’s High School (No. 2016B026) the Clinical Key Subject (Neurosurgery) Foundation of Fujian Medical University the Key Department (Neurosurgery) Foundation from Fujian Medical University Union Hospital,China 

主　　题：aquaporin 4 baicalin Chinese medicine early brain injury matrix metalloproteinase-9 subarachnoid hemorrhage 

摘      要：Objective: To evaluate the effect of baicalin on subarachnoid hemorrhage(SAH) in rats and explore the potential mechanisms. Methods: Sprague-Dawley rats underwent experimental SAH and received treatment with baicalin at 10 or 50 mg/kg after 2 and 12 h of SAH. Neurological scores, brain water content, Evans-blue extravasation, and levels of glutathione peroxidase(GSH-Px), superoxide dismutase(SOD), myeloperoxidase(MPO), and malondialdehyde(MDA) were measured 24 h after SAH. Expression of nuclear factor erythroid-related factor 2(Nrf2), NAD(P)H: quinone oxidoreductase 1(NQO1), matrix metalloproteinase-9(MMP-9), aquaporin 4(AQP4), occludin, and zonulaoccludens-1(ZO-1) were detected in the brain by Western blot. Heme oxygenase-1(HO-1) was detected by quantitative polymerase chain reaction, and tumor necrosis factor-α(TNF-α) and interleukin-1β(IL-1β) were assessed by enzyme-linked immunosorbent assay. Results: Baicalin attenuated EBI 24 h after SAH in rats(P0.05). Baicalin elevated neurological scores, GSH-Px, SOD, and increased the expression of Nrf2, NQO1, HO-1, occludin, and ZO-1 in SAH rats(P0.05 or P0.01). Baicalin reduced MPO, MDA, and the expression of MMP-9, AQP4, TNF-α, and IL-1β(P0.05 or P0.01). Conclusion: Baicalin reduced SAH-induced EBI, partially via activation of the Nrf2/HO-1 pathway and inhibition of MMP-9 and AQP4.