Graphene oxide as a photocatalytic nuclease mimicking nanozyme for DNA cleavage
作者机构：Department of ChemistryWaterloo Institute for NanotechnologyWaterlooOntarioN2L 3G1Canada State Key Laboratory of Hydraulics and Mountain River EngineeringAnalytical&Testing CenterSichuan UniversityChengdu 610064China
出 版 物：《Nano Research》 (纳米研究（英文版）)
年 卷 期：2020年第13卷第2期
页 面：455-460页
核心收录：
学科分类：0808[工学-电气工程] 081704[工学-应用化学] 0809[工学-电子科学与技术（可授工学、理学学位）] 07[理学] 070304[理学-物理化学(含∶化学物理)] 08[工学] 0817[工学-化学工程与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0702[理学-物理学]
基 金：Funding for this work was from the Natural Sciences and Engineering Research Council of Canada(NSERC) the National Natural Science Foundation of China(No.U19A2005)
主 题：graphene oxide photocatalysis nuclease mimicking nanozyme DNA cleavage
摘 要：Developing nanomaterial-based enzyme mimics for DNA cleavage is an interesting challenge and it has many potential ***-layered graphene oxide(GO)is an excellent platform for DNA *** addition,GO has been employed for photosensitized generation of reactive oxygen species(ROS).Herein,we demonstrate that GO sheets could cleave DNA as anuclease mimicking nanozyme in the presence of UV or blue *** various DNA sequences and lengths,well-defined product bands were observed along with photobleaching of the fluorophore label on the *** from previously reported GO cleavage of DNA,our method did not require metal ions such as Cu^2+.Fluorescence spectroscopy suggested a high adsorption affinity between GO and *** comparison,although zero-dimensional fluorescent carbon dots(C-dots)had higher photosensitivity ir terms of producing ROS,their cleavage activity was much lower and only smeared cleavage products were observed,indicatingthat the ROS acted on the DNA in *** on the results,GO behaved like a classic heterogeneous catalyst following substrate adsorption,reaction,and product desorption *** simple strategy may help in the design of new nanozymes by introducing light.
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