Mimicking peroxidase active site microenvironment by functionalized graphene quantum dots

作     者：Qi Xin Xinrui Jia Asmat Nawaz Wenjing Xie Litao Li Jian Ru Gong Qi Xin;Xinrui Jia;Asmat Nawaz;Wenjing Xie;Litao Li;Jian Ru Gong

作者机构：CAS Center of Excellence for NanoscienceCAS Key Laboratory for Nanosystem&Hierarchical FabricationNational Center for Nanoscience and Technology11 Beiyitiao ZhongguancunBeijing 100190China University of Chinese Academy of SciencesBeijing 100049China Department of Orthopaedicsthe 309th Hospital of the PLABeijing 100091China 

出 版 物：《Nano Research》 (纳米研究（英文版）)

年 卷 期：2020年第13卷第5期

页      面：1427-1433页

核心收录：

学科分类：081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 

基　　金：The authors acknowledge financial support for this work from the National Key R&D Program"nanotechnology"special focus(No.2016YFA0201600) the National Natural Science Foundation of China(Nos.21422303,21573049,21872043,81602643) Beijing Natural Science Foundation(No.2142036) the Knowledge Innovation Program,Youth Innovation Promotion Association,and Special Program of"One Belt One Road"of CAS.The authors thank Dr.Dexing Li for the technique support and many helpful discussions for the ITC testing 

主　　题：nanoenzyme graphene quantum dot heme active site peroxidase mimetic enzymatic microenvironment 

摘      要：The development of high-efficiency peroxidase mimetics is highly desirable in view of high cost and low stability of natural *** the perspective of mimicking active site microenvironment at low cost,we herein report a novel histidine-functionalized graphene quantum dot(His-GQD)/hemin complex,which exhibits the highest catalytic rate for the peroxidase-based chromogenic reaction among the hemin-containing mimetics reported so ***,our peroxidase mimetic shows excellent tolerance to strongly acidic conditions and can function in a wide temperature ***-Burk plots and comprehensive electron paramagnetic resonance analysis reveal a ping-pong type catalytic mechanism for this *** addition,His-GQD/hemin demonstrates high efficiency and accuracy in detecting H2O2 and blood *** work provides an effective design of artificial enzymes for practical applications.