Single-cell transcriptomic analysis of adult mouse pituitary reveals sexual dimorphism and physiologic demand-induced cellular plasticity

作     者：Yugong Ho Peng Hu Michael T.Peel Sixlng Chen Pablo G.Camara Douglas J.Epstein Hao Wu Stephen A.Liebhaber Yugong Ho;Peng Hu;Michael T.Peel;Sixlng Chen;Pablo G.Camara;Douglas J.Epstein;Hao Wu;Stephen A.Liebhaber

作者机构：Departments of GeneticsPerelman School of MedicineUniversity of PennsylvaniaPhiladelphiaPA 19104USA Penn Epigenetics InstitutePerelman School of MedicineUniversity of PennsylvaniaPhiladelphiaPA 19104USA Penn Institute for Biomedical InformaticsPerelman School of MedicineUniversity of PennsylvaniaPhiladelphiaPA 19104USA Departments of MedicinePerelman School of MedicineUniversity of PennsylvaniaPhiladelphiaPA 19104USA 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2020年第11卷第8期

页      面：565-583页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：This work was supported by National Institutes of Health(NIH)Grants DK107453(to S.L and Y.H)and R00HG007982 DP2HL142044 and a University of Pennsylvania Epigenetics Institute Pilot Grant(to H.W) 

主　　题：mouse pituitary cellular plasticity sexual dimorphism single-cell RNA sequencing 

摘      要：The anterior pituitary gland drives highly conserved physiologic processes in mammalian *** hormonally controlled processes are central to somatic growth,pubertal transformation,fertility,lactation,and *** cellular models of mammalian anteiror pituitary,largely built on candidate gene based immuno-histochemical and mRNA analyses,suggest that each of the seven hormones synthesized by the pituitary is produced by a specific and exclusive cell ***,emerging evidence suggests more complex relationship between hormone specificity and cell *** we have applied massively parallel single-cell RNA sequencing (scRNA-seq),in conjunction with complementary imaging-based single-cell analyses of mRNAs and proteins,to systematically map both cell-type diversity and functional state heterogeneity in adult male and female mouse pituitaries at single-cell resolution and in the context of major physiologic *** quantitative single-cell analyses reveal sex-specific cell-type composition under normal pituitary homeostasis,identify an array of cells associated with complex complements of hormone-enrlchment,and undercover non-hormone producing interstitial and supporting ***,we also identified a Pou1f1-expressing cell population that is characterized by a unique multi-hormone gene expression *** response to two well-defined physiologic stresses,dynamic shifts in cellular diversity and trenscrlptome profiles were observed for major hormone producing and the putative multi-hormone *** studies reveal unanticipated cellular complexity and plasticity in adult pituitary,and provide a rich resource for further validating and expanding our molecular understanding of pituitary gene expression programs and hormone production.