UGT74AF3 enzymes specifically catalyze the glucosylation of 4-hydroxy-2,5-dimethylfuran-3(2H)-one,an important volatile compound in Camellia sinensis

作     者：Yongxian Chen Xiangyang Guo Ting Gao Na Zhang Xiaochun Wan Wilfried Schwab Chuankui Song Yongxian Chen;Xiangyang Guo;Ting Gao;Na Zhang;Xiaochun Wan;Wilfried Schwab;Chuankui Song

作者机构：State Key Laboratory of Tea Plant Biology and UtilizationInternational Joint Laboratory on Tea Chemistry and Health EffectsAnhui Agricultural University230036 HefeiAnhuiP.R.China Biotechnology of Natural ProductsTechnische Universität MünchenLiesel-Beckmann-Str.185354 FreisingGermany 

出 版 物：《Horticulture Research》 (园艺研究（英文）)

年 卷 期：2020年第7卷第1期

页      面：2433-2443页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1001[医学-基础医学(可授医学、理学学位)] 09[农学] 0901[农学-作物学] 0703[理学-化学] 090203[农学-茶学] 0836[工学-生物工程] 0902[农学-园艺学] 0834[工学-风景园林学（可授工学、农学学位）] 

基　　金：supported by the National Natural Science Foundation of China(31961133030,31870678) National Key Research and Development Program of China(2018YFD1000601) Science Fund for Distinguished Young Scientists of Anhui Province(1908085J12) 

主　　题：metabolism volatile sugar 

摘      要：4-Hydroxy-2,5-dimethylfuran-3(2H)-one(HDMF)is an important odorant in some fruits,and is proposed to play a crucial role in the caramel-like notes of some ***,its biosynthesis and metabolism in tea plants are still ***,HDMF glucoside was unambiguously identified as a native metabolite in tea plants.A novel glucosyltransferase UGT74AF3a and its allelic protein UGT74AF3b specifically catalyzed the glucosylation of HDMF and the commercially important structural homologues 2(or 5)-ethyl-4-hydroxy-5(or 2)-methylfuran-3(2H)-one(EHMF)and 4-hydroxy-5-methylfuran-3(2H)-one(HMF)to their correspondingβ-***-directed mutagenesis of UGT74AF3b to introduce a single A456V mutation resulted in improved HDMF and EHMF glucosylation activity and affected the sugar donor preference compared with that of the wild-type control *** accumulation of HDMF glucoside was consistent with the transcript levels of UGT74AF3 in different tea *** addition,transient UGT74AF3a overexpression in tobacco significantly increased the HDMF glucoside contents,and downregulation of UGT74AF3 transcripts in tea leaves significantly reduced the concentration of HDMF glucoside compared with the levels in the *** identification of HDMF glucoside in the tea plant and the discovery of a novel-specific UDP-glucose:HDMF glucosyltransferase in tea plants provide the foundation for improvement of tea flavor and the biotechnological production of HDMF glucoside.