MicroRNA-29a attenuates angiotensin-Ⅱ induced-left ventricular remodeling by inhibiting collagen,TGF-β and SMAD2/3 expression

作     者：Si-Jin ZHANG Cui-Juan YUN Jie LIU Si-Yu YAO Yao LI Miao WANG Chi WANG Yong-Yi BAI Hao XUE Si-Jin ZHANG;Cui-Juan YUN;Jie LIU;Si-Yu YAO;Yao LI;Miao WANG;Chi WANG;Yong-Yi BAI;Hao XUE

作者机构：Department of CardiologyChinese People’s Liberation Army General HospitalBeijingChina Department of Geriatric CardiologyChinese People’s Liberation Army General HospitalBeijingChina 

出 版 物：《Journal of Geriatric Cardiology》 (老年心脏病学杂志（英文版）)

年 卷 期：2020年第17卷第2期

页      面：96-104页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(No.81570383) 

主　　题：Left ventricular remodeling MicroRNA-29a SMAD2/3 Transforming growth factor-β 

摘      要：Background Left ventricular(LV)remodeling is the most common target organ damage in ***,our study found that plasma microRNA-29a(miR-29a)level was associated with the LV remodeling in hypertensive ***,the causal relationship between miR-29a and LV remodeling remains ***,the aim of this study was to investigate the regulation mechanism of miR-29a in LV ***&Results Overexpression and knockdown miR-29a mice were generated by tail-intravenous injection of miR-29a-mimic and inhibitor lentivirus for one week *** the mice were subjected to angiotensin-II(AngII)induced LV remodeling by subcutaneous AngII capsule osmotic pumping into AngII for four ***-induced LV remodeling mice as the model group(n=9).Age-matched male SPF C57/BL6J mice(6–8 weeks old)were treated with the pumping of saline as a vehicle(n=6).In vivo,overexpression miR-29a ameliorated AngII-induced LV remodeling,while knockdown miR-29a deteriorated LV ***,we observed that overexpression miR-29a mice inhibited but knockdown miR-29a mice increased cardiac cross-sectional area,indicating that miR-29a has an antagonistic effect on cardiac *** studies found that overexpression miR-29a inhibited the content of the LV collagen including collagen I and ***,the expression of transforming growth factor-β(TGF-β)and phosphorylated SMAD2/3 decreased with the down-regulation of collagen I and III in overexpression miR-29a *** Our finding indicates that overexpression miR-29a attenuates LV remodeling by inhibiting collagen deposition,TGF-β,and phosphorylated SMAD2/3 ***,intervention miR-29a may be a therapeutic target for attenuating LV remodeling.