Follicle-stimulating hormone promotes proliferation of cultured chicken ovarian germ cells through protein kinases A and C activation

作     者：Hong-yun LIU Wei-dong ZENG Ai-ling CAO Cai-qiao ZHANG 

作者机构：Key Laboratory of Animal Epidemic Etiology and lmmunological Prevention of the Ministry of Agriculture College of Animal Sciences Zhejiang University Hangzhou 310029 China Xiaoshan Entry-Exit Inspection and Quarantine Bureau Hangzhou 311208 China 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2010年第11卷第12期

页      面：952-957页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：Project supported by the National Natural Science Foundation of China (No. 30871843) the Key Project of the Science and Technology Department of Zhejiang Province, China (No. 2008C12050) 

主　　题：Follicle-stimulating hormone Protein kinase A Protein kinase C Germ cell Proliferation 

摘      要：The study was conducted to investigate the effects of follicle-stimulating hormone (FSH) on embryonic chicken ovarian germ cell proliferation and its possible involvements of protein kinases A (PKA) and C (PKC) *** cells were treated with FSH alone or in the presence of forskolin (FRSK),PKA inhibitor (H89),PKC activator (PMA) or inhibitor (H7).The germ cell number was counted from *** immunocytochemistry of proliferating cell nuclear antigen (PCNA) was applied to identify the proliferating *** germ cell labeling index (LI) was determined for cell *** FSH treatment increased the germ cell number,and this stimulating effect was enhanced by FRSK or PMA,but inhibited by H89 or H7 in a dose-dependent ***,the PCNA-LI showed parallel changes with germ cell *** study suggests that FSH may stimulate proliferation of cultured chicken ovarian germ cells by activation of both the PKA and PKC signaling pathways.