Thrombospondin-1 Expression in RPE and Choroidal Neovascular Membranes

作     者：Shikun He Francesca Incardona Manlin Ji Stephen J. Ryan David R. Hinton 

作者机构：Departments of Patholog Keck School of Medicine of the University of Southern Cdifornia Ophthalmology Keck School of Medicine of the University of Southern Cdifornia Doheny Eye Institute Los A ngeles CA 

出 版 物：《眼科学报》 (Eye Science)

年 卷 期：2006年第22卷第4期

页      面：265-274页

学科分类：10[医学] 

主　　题：thrombospondin 1 retinal pigment epithelium choroidal neovascular membrane 

摘      要：Purpose: To investigate the expression of thrombospondin 1 (TSP-1) in retinal pigment epithelium (RPE) and choroidal neovascular membranes (CNVMs) from patients with age-related macular degeneration (AMD). Methods: Tissue sections from normal human fetal and adult eyes and surgically removed CNVMs were immunostained for TSP-1 localization. Polymerase chain reaction and Western blotting were used to analyze TSP-1 mRNA and protein from human RPE cells, respectively. TSP-1 in the supernatant of cultured RPE cells and eye explants were measured using enzyme-linked immunosorbent assay. MTT assay was used to evaluate the RPE survival after TSP-1 treatment. Results: The strongest immunostaining for TSP-1 was observed in the RPE monolayer around drusen in early AMD. The intensity of TSP-1 staining in normal eye sections was much weaker than that of early AMD and CNVM. TSP-1 mRNA was positive in cultured fetal and adult RPE cells. There was increasing secretion of TSP-1 into the supernatant of cultured RPE and eye explants. The specific band of TSP-1 was identified by Western blot. No significant inhibition of RPE survival was found with the exposure to TSP-1. Conclusions: TSP-1 expression in drusen and CNVM was upregulated and associated with RPE monolayer. TSP-1 may be a natural negative regulator for choroidal neovascularization.