Red carbon dots as label-free two-photon fluorescent nanoprobes for imaging of formaldehyde in living cells and zebrafishes

作     者：Hong Wang Jing Wei Chonghua Zhang Yuan Zhang Yubin Zhang Lin Li Changmin Yu Peisheng Zhang Jian Chen Hong Wang;Jing Wei;Chonghua Zhang;Yuan Zhang;Yubin Zhang;Lin Li;Changmin Yu;Peisheng Zhang;Jian Chen

作者机构：Key Laboratory of Theoretical Organic Chemistry and Functional Molecule of Ministry of EducationHunan Provincial Key Laboratory of Controllable Preparationand Functional Application of Fine PolymersHunan Provincial Key Lab of Advanced Materials for New Energy Storage and ConversionHunan Province College KeyLaboratory of QSAR/QSPRSchool of Chemistry and Chemical EngineeringHunan University of Science and TechnologyXiangtan 411201China Key Laboratory of Flexible Electronics(KLOFE)&Institute of Advanced Materials(IAM)Nanjing Tech UniversityNanjing 211800China State Key Laboratory of Luminescent Materials and DevicesSouth China University of TechnologyGuangzhou 510640China 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2020年第31卷第3期

页      面：759-763页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070205[理学-凝聚态物理] 070302[理学-分析化学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0702[理学-物理学] 

基　　金：financial support of the present work by the National Natural Science Foundation of China(Nos.51773056,51603067,21705040) Natural Science Foundationof Hunan Province,China(No.2018JJ3143) China Postdoctoral Science Foundation(Nos.2017M622571,2017M622568 and 2018T110824) Open Project Program of State Key Laboratory of Chemo/Biosensing and Chemometrics(No.2016019) Open Fund of the State Key Laboratory of Luminescent Materials and Devices(South China University of Technology)(No.2019-skllmd-09) Natural Science Foundation of Guangdong Province(No.2017A030313299) 

主　　题：Carbon dots(CDs) Two-photon excitation Formaldehyde(FA) Intracellular imaging Zebrafishes 

摘      要：Direct,in situ selective detection of intracellular formaldehyde(FA)is of great significance for understanding its function in FA-related ***,red carbon dots(RCD)are reported as label-free two-photon fluorescent nanoprobes for detecting and imaging of *** addition of FA,the-NH2 groups of RCD could quickly and specially react with aldehydes to form Schiff base and then the strong fluorescence of RCD with blue-shift emission is recovery due to the destruction of the hydrogen bond interaction between RCD and *** addition,the nanoprobes exhibit outsta nding photo stability,rapid response(1 min),high sensitivity(~9.9μmol/L)and excellent selectivity toward FA over other aldehyde group ***,owing to the good cell-membrane permeability and biocompatibility,as well as the large two-photon absorption cross-section,the as-prepared RCD can be used as label-free nanoprobes for selectively detecting and imaging FA in living cells and zebrafishes through one-photon and two-photon ***,RCD could stain the tissue of zebrafishes at depths interval of up to 240μm under two-photon *** research implied that RCD are promising tools for directly and in situ imaging FA in vivo,thus providing critical insights into FA-related pathophysiological processes.