Correlation network analysis shows divergent effects of a long-term,high-fat diet and exercise on early stage osteoarthritis phenotypes in mice

作     者：Timothy M.Griffin Albert Batushansky Joanna Hudson Erika Barboza Prado Lopes Timothy M. Griffin;Albert Batushansky;Joanna Hudson;Erika Barboza Prado Lopes

作者机构：Aging and Metabolism Research ProgramOklahoma Medical Research Foundation(OMRF)Oklahoma CityOK 73104USA Reynolds Oklahoma Center on Aging and Departments of Biochemistry and Molecular BiologyPhysiologyand Geriatric MedicineUniversity of Oklahoma Health Sciences CenterOklahoma CityOK 73104USA 

出 版 物：《Journal of Sport and Health Science》 (运动与健康科学（英文）)

年 卷 期：2020年第9卷第2期

页      面：119-131页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by the National Institutes of Health(P20RR018758,P20GM103441,P30GM114731,R03AR066828,and R01AG049058 to Dr.Griffin) the Arthritis Foundation(Arthritis Investigator Award to Dr.Griffin) 

主　　题：Inflammation Knee Mouse Obesity Wheel running 

摘      要：Background:Obesity increases knee osteoarthritis(OA) risk through metabolic,inflammatory,and biomechanical factors,but how these systemic and local mediators interact to drive OA pathology is not well *** tested the effect of voluntary running exercise after chronic diet-induced obesity on knee OA-related cartilage and bone pathology in *** then used a correlation-based network analysis to identify systemic and local factors associated with early-stage knee OA phenotypes among the different diet and exercise ***:Male C57 BL/6 J mice were fed a defined control(10% kcal fat) or high fat(HF)(60% kcal fat) diet from 6 to 37 weeks of *** 25 weeks,one-half of the mice from each diet group were housed in cages with running wheels for the remainder of the ***,micro computed tomography,and magnetic resonance imaging were used to evaluate changes in joint tissue structure and OA *** local variables were then compared to systemic metabolic(body mass,body fat,and glucose tolerance),inflammatory(serum adipokines and inflammatory mediators),and functional(mechanical tactile sensitivity and grip strength) outcomes using a correlation-based network *** and exercise effects were evaluated by two-way analysis of ***:An HF diet increased the infrapatellar fat pad size and posterior joint osteophytes,and wheel running primarily altered the subchondral cortical and trabecular *** HF diet nor exercise altered average knee cartilage OA scores compared to control ***,the coefficient of variation was≥25% for many outcomes,and some mice in both diet groups developed moderate OA(33% maximum score).This supported using correlation-based network analyses to identify systemic and local factors associated with early-stage knee OA *** wheel-running cohorts,an HF diet reduced the network size compared to the control diet group despite similar running distances,suggesting that diet-induced obesi