Effects of tetrahedral framework nucleic acid/wogonin complexes on osteoarthritis

作     者：Sirong Shi Yang Chen Taoran Tian Songhang Li Shiyu Lin Yuxin Zhang Xiaoru Shao Tao Zhang Yunfeng Lin Xiaoxiao Cai Sirong Shi;Yang Chen;Taoran Tian;Songhang Li;Shiyu Lin;Yuxin Zhang;Xiaoru Shao;Tao Zhang;Yunfeng Lin;Xiaoxiao Cai

作者机构：State Key Laboratory of Oral DiseaseNational Clinical Research Center for Oral DiseasesWest China Hosptial of StomatologySichuan UniversityChengdu 610041China Department of Liver Surgery&Liver Transplantation CenterWest China Hospital of Sichuan UniversityChengdu 610041 Sichuan ProvinceChina 

出 版 物：《Bone Research》 (骨研究（英文版）)

年 卷 期：2020年第8卷第1期

页      面：84-96页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：supported by National Key R&D Program of China (2019YFA0110600) National Natural Science Foundation of China (81970986, 81771125) 

主　　题：complexes tetrahedral promoted 

摘      要：Osteoarthritis, a disorder characterized by articular cartilage deterioration, varying degrees of inflammation, and chondrocyte apoptosis, is the most common chronic joint disease. To slow or reverse its progression, inflammation should be inhibited, and chondrocyte proliferation should be promoted. Tetrahedral framework nucleic acids can be internalized by chondrocytes(even inflammatory chondrocytes) and can enhance their proliferation and migration. Wogonin, a naturally occurring flavonoid,suppresses oxidative stress and inhibits inflammation. In this study, tetrahedral framework nucleic acids were successfully selfassembled and used to load wogonin. We confirmed the effective formation of tetrahedral framework nucleic acid/wogonin complexes by dynamic light scattering, zeta potential analysis, transmission electron microscopy, and fluorescence spectrophotometry. Tetrahedral framework nucleic acids, wogonin, and especially tetrahedral framework nucleic acid/wogonin complexes effectively alleviated inflammation in vitro and in vivo and prevented cartilage destruction. In addition, these materials remarkably downregulated the expression of inflammatory mediators and matrix metalloproteinases, upregulated chondrogenic markers, and promoted tissue inhibitor of metalloproteinase 1 and B-cell lymphoma 2 expression. In vivo, after treatment with tetrahedral framework nucleic acid/wogonin complexes, the bone mineral density in regenerated tissues was much higher than that found in the untreated groups. Histologically, the complexes enhanced new tissue regeneration, significantly suppressed chondrocyte apoptosis, and promoted chondrogenic marker expression. They also inhibited cell apoptosis, increased chondrogenic marker expression, and suppressed the expression of inflammatory mediators in osteoarthritis. Therefore, we believe that tetrahedral framework nucleic acid/wogonin complexes can be used as an injectable form of therapy for osteoarthritis.