Temporomandibular joint damage in K/BxN arthritic mice

作     者：Sabine Kuchler-Bopp Alexandre Mariotte Marion Strub Chrystelle Po Aurore De Cauwer Georg Schulz Xavier Van Bellinghen Florence Fioretti Francois Clauss Philippe Georgel Nadia Benkirane-Jessel Fabien Bornert Sabine Kuchler-Bopp;Alexandre Mariotte;Marion Strub;Chrystelle Po;Aurore De Cauwer;Georg Schulz;Xavier Van Bellinghen;Florence Fioretti;Fran?ois Clauss;Philippe Georgel;Nadia Benkirane-Jessel;Fabien Bornert

作者机构：INSERM(French National Institute of Health and Medical Research)UMR 1260Regenerative NanoMedicine(RNM)FMTSStrasbourgFrance INSERMUMR 1109Immuno Rhumatologie Moléculaire(IRM)FMTSStrasbourgFrance Fédération Hospitalo-Universitaire(FHU)OMICAREUniversitéde StrasbourgStrasbourgFrance Facultéde Chirurgie DentaireUniversitéde Strasbourg(UDS)8 rue Ste ElisabethStrasbourgFrance Pole de Médecine et Chirurgie Bucco-DentairesHopitaux Universitaires de Strasbourg(HUS)1 place de l’HopitalStrasbourgFrance ICube UMR 7357Universitéde StrasbourgCNRSFMTS4 rue KirschlegerStrasbourgFrance Department of Biomedical EngineeringCore Facility Micro-and NanotomographyBiomaterials Science Center(BMC)University of BaselGewerbestrasse 144123AllschwilSwitzerland 

出 版 物：《International Journal of Oral Science》 (国际口腔科学杂志（英文版）)

年 卷 期：2020年第12卷第1期

页      面：59-67页

核心收录：

学科分类：1003[医学-口腔医学] 100302[医学-口腔临床医学] 10[医学] 

基　　金：This work was funded by the INSERM 

主　　题：damage alterations inflammation 

摘      要：Rheumatoid arthritis(RA) is an autoimmune disease affecting 1% of the world population and is characterized by chronic inflammation of the joints sometimes accompanied by extra-articular manifestations. K/Bx N mice, originally described in 1996 as a model of polyarthritis, exhibit knee joint alterations. The aim of this study was to describe temporomandibular joint(TMJ)inflammation and damage in these mice. We used relevant imaging modalities, such as micro-magnetic resonance imaging(μMRI)and micro-computed tomography(μCT), as well as histology and immunofluorescence techniques to detect TMJ alterations in this mouse model. Histology and immunofluorescence for Col-I, Col-II, and aggrecan showed cartilage damage in the TMJ of K/Bx N animals, which was also evidenced by μCT but was less pronounced than that seen in the knee joints. μMRI observations suggested an increased volume of the upper articular cavity, an indicator of an inflammatory process. Fibroblast-like synoviocytes(FLSs)isolated from the TMJ of K/Bx N mice secreted inflammatory cytokines(IL-6 and IL-1β) and expressed degradative mediators such as matrix metalloproteinases(MMPs). K/Bx N mice represent an attractive model for describing and investigating spontaneous damage to the TMJ, a painful disorder in humans with an etiology that is still poorly understood.