Expression and contribution of microphthalmia-associated transcription factor to the melanin deposition in Liancheng white ducks

作     者：XIN Qing-wu MIAO Zhong-wei LIU Zhao-yuan LI Li ZHANG Lin-li ZHU Zhi-ming ZHANG Zhenghong ZHENG Nen-zhu WANG Zheng-chao XIN Qing-wu;MIAO Zhong-wei;LIU Zhao-yuan;LI Li;ZHANG Lin-li;ZHU Zhi-ming;ZHANG Zheng-hong;ZHENG Nen-zhu;WANG Zheng-chao

作者机构：Institute of Animal Husbandry and Veterinary MedicineFujian Academy of Agricultural SciencesFuzhou 350013P.R.China Provincial Key Laboratory for Developmental Biology and Neurosciences/Key Laboratory of OptoElectronic Science and Technology for Medicine of Ministry of EducationCollege of Life SciencesFujian Normal UniversityFuzhou 350007P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2020年第19卷第3期

页      面：800-809页

核心收录：

学科分类：090603[农学-临床兽医学] 09[农学] 0906[农学-兽医学] 

基　　金：supported by the National Natural Science Foundation of China (31372474) the National Guidance Project for Local Science and Technology Development,China (2018L3004) the National and FNU Training Program of Innovation and Enterpreneurship for Undergraduates, China (201910394023, CXXL2019268 and CXXL2019274) the Fujian Province Public Welfare Scientific Project, China (018R1023-2) the Fujian Development and Reform Commission “Five News” Project of China (fjfgw201806) the Young Talent Innovation Funding of Fujian Academy of Agricultural Sciences of China (YC2017-7 and MYQJ2015-5) 

主　　题：microphthalmia-associated transcription factor genetic evolution analysis melanin deposition Liancheng white ducks 

摘      要：The present study investigates the expression of microphthalmia-associated transcription factor(MITF) and its contribution to the melanin deposition in Liancheng white *** PCR was used to clone the MITF gene sequence from the skin tissue of female Liancheng white *** spectrophotometry was used to detect the melanin *** mRNA expression and melanin deposition in different tissues and organs were detected and their correlation was *** MITF gene(GenBank number: MG516570) was 1 323 bp in length,contains a complete CDS region(34-1 323 bp) and codes 429 amino acids with 100% homology to the MITF of Anas platyrhynchos and over 95% homology to those of Gallus gallus and Coturnix *** evolution analysis reveals a close relationship of Liancheng white ducks with ***,and also to lesser extents with Anser cygnoides,silky fowl and ***,as well as Sus scrofa,Ovis aries and other ***-time quantitative PCR(qPCR) analysis demonstrated that MITF was expressed in skin,gizzard,liver,kidney and muscle,and of these tissues,its expression was the highest in the skin tissue(skingizzardliverkidneymuscle).Ultraviolet spectrophotometry showed that melanin deposition was positively correlated with the MITF expression level in these five tissues and organs(P0.05).Together,these results demonstrated a tissue-specific pattern of MITF expression and a positive correlation between MITF expression and melanin deposition,indicating that MITF expression may contribute to the melanin deposition in Liancheng white ducks.