Effects of plant-derived analgesic compounds sinomenine and salvinorin A in infant rats

作     者：Conrad J.Mascarenhas Renyu Liu Gordon A.Barr Conrad J.Mascarenhas;Renyu Liu;Gordon A.Barr

作者机构：Department of Anesthesiology and Critical CareChildren’s Hospital of PhiladelphiaPhiladelphiaPA 19104USA Department of Anesthesiology and Critical CarePerelman School of Medicine at the University of PennsylvaniaPhiladelphiaPA 19104USA Department of PsychologyUniversity of PennsylvaniaPhiladelphiaPA 19104USA 

出 版 物：《Journal of Integrative Medicine》 (结合医学学报（英文版）)

年 卷 期：2020年第18卷第2期

页      面：174-180页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported in part by the Department of Anesthesiology and Critical Care at the Children’s Hospital of Philadelphia,the James Battaglia Endowed Chair to G.A.Barr a Faculty Mentoring Undergraduate Research grant to C.J.Mascarenhas and G.A.Barr supported by National Institutes of Health Grant R01(1R01GM111421) the Department of Anesthesiology and Critical Care at the University of Pennsylvania 

主　　题：Thermal pain Formalin test Fos Ontogeny Sinomenine Salvinorin A 

摘      要：Objective: Premature and ill neonates undergo painful but medically necessary procedures while hospitalized. Although opiate drugs are administered as analgesics, problems associated with their side effects,tolerance, and potential dependence necessitate research into alternative pain-relieving *** we test two plant-derived compounds in infant rats: sinomenine, which targets the Mas-related G-protein-coupled receptor member X2 opioid receptor;and salvinorin A, which is a j opioid receptor agonist. In adult animals both sinomenine and salvinorin A are analgesic, but neither has been tested in ***: We used the formalin and thermal plantar tests in rats 7 and 21 days of age(PN7 and PN21) for behavioral signs of pain. In addition, brain sections were stained using Fos immunohistochemistry to examine patterns of brain activation in the midbrain periaqueductal gray and the paraventricular nucleus of the ***: Sinomenine was analgesic in both the formalin and thermal tests on animals 21 days of age. At PN7 only the highest dose elevated response latencies in the thermal test and there were no effects of sinomenine in the formalin test. Analysis of Fos expression in the sinomenine-treated animals showed no drug effect, in contrast to the behavioral results. Salvinorin A was analgesic in the formalin test only at the highest dose at 21 days of age but not in the thermal test at either ***: The increased modest effectiveness of sinomenine in older animals and the minimum salvinorin A drug effect suggest that the compounds act on sites that develop during the preweaning period(sinomenine) or after weaning(salvinorin A).