Study on neuronal apoptosis induced by hypoxia or traumatic injury in rats

作     者：姜曙 于如同 鞠延 贺民 毛伯镛 

作者机构：Department of Neurosurgery First Affiliated Hospital West China University of Medical Sciences Chengdu 610041 China. 

出 版 物：《Chinese Journal of Traumatology》 (中华创伤杂志（英文版）)

年 卷 期：2000年第3卷第3期

页      面：149-152页

学科分类：1001[医学-基础医学(可授医学、理学学位)] 100104[医学-病理学与病理生理学] 10[医学] 

主　　题：Apoptosis Anoxia Wounds and injuries Gene expression 

摘      要：Objective: To explore the relationship between neuronal apoptosis and hypoxia or traumatic injury. Methods: Rat neurons primarily cultured in vitro were treated with hypoxia (the hypoxia group) or traumatic injury (the trauma group). The neuronal apoptosis was evaluated with microscope, TUNEL (terminal deoxynucleotidyl transferase mediated X dUTPnick end labeling) staining, flow cytometry, agarose gel electrophoresis and immunohistochemistry. Results: Morphological changes of apoptosis appeared in the treated neurons,and the DNA fragmentation showed “ladder break. The apoptotic index was 10.8% in the hypoxia group and 4.8% in the trauma group, while it was only 1.6% in the control group. The expression of apoptosis associated genes (c myc, fas and fasL) ***: Hypoxia or traumatic injury can induce neuronal apoptosis, and its molecular mechanism is probably related to the expressions of apoptosis associated genes.