Global DNA methylation and transcriptional analyses of human ESC-derived cardiomyocytes

作     者：Ying Gut Guang-Hui Liu Nongluk Plongthongkum' Christopher Benner Fei Yi Jing Qu Keiichiro Suzuki Jiping Yang Weiqi Zhang Mo Li Nuria Montserrat Isaac Crespo Antonio del Sol Concepcion Rodriguez Esteban Kun Zhang Juan Carlos Izpisua Belmonte 

作者机构：Gene Expression Laboratory Salk Institute for Biological Studies 10010 North Torrey Pines Road La Jolla CA 92037 USA National Laboratory of Biomacromolecules institute of Biophysics Chinese Academy of Sciences Beijing 100101 China ' State key Laboratory of Drug Research Shanghai Institute of Materia Medica Chinese Academy of Sciences Shanghai 201203 China Department of Bioengineering University of California at San Diego La Jolla CA 92093 USA center of Regenerative Medicine in Barcelona Dr. Aiguader 88 08003 Barcelona Spain Biomedical Research Networking Center in Bioengineering Biomaterials and Nanomedicine (CIBER-BBN) Poeta MarianoEsquillor s/n 50018 Sargossa Spain Luxembourg Centre for Systems Biomedicine (LCSB) University of Luxembourg Walferdange Luxembourg 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2014年第5卷第1期

页      面：59-68页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0703[理学-化学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：N I H supported by a CIRM training grant. K.Z is supported by California Institute for Regenerative Medicine the National Laboratory of Biomacromolecules, and the State Key Laboratory of Drug Research 国家自然科学基金 国家973计划 supported by the Strategic Priority Research Program of the Chinese Academy of Sciences supported by the NationalBasicResearchProgram(973Program) 

主　　题：human cardiomyocyte DNA methylation,microarray heart development 

摘      要：With defined culture protocol, human embryonic stem cells （hESCs） are able to generate cardiomyocytes in vitro, therefore providing a great model for human heart development, and holding great potential for car- diac disease therapies. In this study, we successfully generated a highly pure population of human cardio- myocytes （hCMs） （〉95% cTnT＋） from hESC line, which enabled us to identify and characterize an hCM-specific signature, at both the gene expression and DNA meth- ylation levels. Gene functional association network and gene-disease network analyses of these hCM-enriched genes provide new insights into the mechanisms of hCM transcriptional regulation, and stand as an informative and rich resource for investigating cardiac gene func- tions and disease mechanisms. Moreover, we show that cardiac-structural genes and cardiac-transcription fac- tors have distinct epigenetic mechanisms to regulate their gene expression, providing a better understandingof how the epigenetic machinery coordinates to regulate gene expression in different cell types.