An in vivo model of in situ implantation using pulmonary valved conduit in large animals under off-pump condition

作     者：WU Hao XU Zhi-wei LIU Xian-min GONG Da WAN Ju-yi XU Xiu-fang ZHOU Zi-fan LI Wen-bin 

作者机构：Department of Cardiac Surgery Institute of Heart Lung and Blood Vessel Disease Beijing Anzhen HospitalCapital Medical University Beijing 100029 China Department of Cardiovascular Surgery First Affiliated Hospital of Soochow University Suzhou Jiangsu 215006 China Department of Thoracic and Cardiovascular Surgery Anyang Chinese Medicine Hospital Anyang Henan 455000 China Department of Molecular Biology Institute of Heart Lung and Blood Vessel Disease Beijing Anzhen HospitalCapital Medical University Beijing 100029 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2013年第126卷第23期

页      面：4540-4544页

核心收录：

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

基　　金：This work was supported by grants from'the National Natural Science Foundation of China (No. 81070077 and No. 81270217)  the Beijing High-level Health Expert Foundation (No. 2009-3-44)  and the Capital Medical University Basic and Clinic Cooperative Foundation (No. 11JL09) 

主　　题：pulmonary valved conduit off-pump juvenile sheep 

摘      要：Background The application of pulmonary valved conduit to reconstruct the continuity between right ventricles and pulmonary artery is one of the major *** study aimed to establish an in vivo model of in situ implantation using pulmonary valved conduit in large animals under off-pump condition to validate the long-term effects of artificial pulmonary valved *** Domesticate juvenile male sheep and tissue-engineered poorine pulmonary valved conduit were used for the experiment：30 sheep,weighing （15±3） kg （range 13 to 17 kg） were randomly divided into two groups which were all operated under general anesthesia by off-pump surgery （group 1） and left thoracotomy （group 2）.Two different off-pump surgical methods were used to perform cannulation in sheep pulmonary artery to replace part of sheep pulmonary artery with pulmonary valved conduit which will work together with sheep pulmonary artery and *** the experiments,animal survival,complication rates,operating time and blood loss were recorded to compare the results between groups and to establish a surgical method with minimal invasion,simplicity,safety,and high success *** In group 1,a total of 15 cases of surgeries were performed,in which two sheep died; the operative mortality was 13.3％ （2/15）.In group 2,a total of 15 cases of surgeries were performed,and the surgical mortality rate was 0 （0/15）.The operation time and blood loss in group 2 was significantly better than that in group *** postoperative echocardiograms showed that,after the surgeries by these two methods,the blood flows were normal,and the valves can open and close *** after 6 months showed that the inner wall and the valves of pulmonary valved conduit were smooth with no thrombus *** These two off-pump methods are feasible and safe with fewer traumas; but the second method is better and particularly suitable for the establishment of a juvenile animal model.