Inhibition of P2X receptor-mediated inward current by protein kinase C in small-diameter dorsal root ganglion neurons of adult rats

作     者：别毕华 张一红 赵志奇 

作者机构：复旦大学神经生物学研究所脑科学研究院国家医学神经生物学重点实验室上海200032 

出 版 物：《Neuroscience Bulletin》 (神经科学通报（英文版）)

年 卷 期：2009年第25卷第4期

页      面：179-186页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071006[理学-神经生物学] 

基　　金：supported by grants from the National Basic Research Development Program of China (No.2006CB500807) the National Natural Science Foundation of China (No.30600178) 

主　　题：dorsal root ganglion P2X ATP β,γ-me-ATP protein kinase C 

摘      要：Objective The aim of the present study is to verify the ATP-induced varied responses in isolated dorsal root ganglion （DRG） neurons of the adult rat, and investigate the modulatory effects of specific P2X receptor agonist β,γ-me-ATP and protein kinase C （PKC） on P2X receptor-mediated inward current in DRG neurons. Methods Whole cell patch-clamp was employed to record the currents on acutely isolated DRG neurons in the adult rats. Results β,γ-me-ATE similar as ATE evoked 2 distinct subtypes of P2X receptor-mediated inward currents in a dose-dependent manner in DRG neurons. Activation of PKC by phorbol 12,13-dibutyrate （PDBu） significantly inhibited both subtypes of inward currents mediated by P2X receptors in a dose-dependent manner. Conclusion Activation of PKC negatively modulated the P2X receptor-mediated currents in rat DRG neurons, which may be of benefit to preventing the over-excitation of nociceptor under inflammatory or neuropathic conditions.