N-cadherin expression in the midbrain substantia nigra of perinatal rats

作     者：Junping Cao Yu Sun Xia Du Dianshuai Gao 

作者机构：Department of Neurobiology Xuzhou Medical College Xuzhou 221002 Jiangsu Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2008年第3卷第10期

页      面：1136-1140页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

基　　金：the National Natural Science Foundation of China No.3570564 

主　　题：N-cadherin substantia nigra nervous system development 

摘      要：BACKGROUND： Cellular adhesion molecule mediates the effects of glial cell line-derived neurotrophic factor in dopaminergic neurons during nervous system development. It is assumed that cellular adhesion molecule plays an important role in dopaminergic neuron development. OBJECTIVE： To investigate N-cadherin expression in the midbrain substantia nigra during rat development. DESIGN, TIME AND SETTING： The developmental biology, controlled study was performed at the Laboratory of Research Center of Neurobiology, Xuzhou Medical College, China from April to October 2007. MATERIALS： Sprague Dawley rats （embryonic days 16, 18, and 20, as well as postnatal days 1, 3, 7, 14, and 21） were selected for this study. N-cadherin antibody was purchased from Santa Cruz, USA. METHODS： N-cadherin expression in the midbrain substantia nigra of perinatal rats was detected using reverse transcription polymerase chain reaction, Western blot, and immunohistochemistry methods. N-cadherin-positive cells were counted. MAIN OUTCOME MEASURES： N-cadherin mRNA, protein, and positive cells in the rat midbrain substantia nigra were quantified. RESULTS： The results of reverse transcription polymerase chain reaction and Western blot demonstrated that altered N-cadherin mRNA levels were similar to protein levels in the midbrain substantia nigra. N-cadherin mRNA and protein expression was low in the midbrain substantia nigra of embryonic day 16 rats but gradually increased, and reached a peak at postnatal day 1. N-cadherin mRNA and protein levels were still high at postnatal days 3 and 7, but significantly decreased at postnatal day 14. There was no significant difference in N-cadherin mRNA and protein expression between postnatal days 14 and 21 （P 〉 0.05）. The immunohistochemistry results demonstrated that there was no significant difference in the quantity of N-cadherin-positive cells per area in rats from embryonic day 16 to postnatal day 7 （P 〉 0.05）. The quantity of N-cadherin-positive cells per a