Diagnostic value of negative enrichment and immune fluorescence in situ hybridization for intraperitoneal free cancer cells of gastric cancer

作     者：Anqiang Wang Zhongwu Li Qian Wang Yali Bai Xin Ji Tao Fu Ke Ji Yanwen Xue Tingxu Han Xiaojiang Wu Ji Zhang Yingjie Yang Guobin Xu Zhaode Bu Jiafu Ji 

作者机构：Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing)Center of Gastrointestinal SurgeryPeking University Cancer Hospital&InstituteBeijing 100142China Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing)Department of PathologyPeking University Cancer Hospital&InstituteBeijing 100142China Cyttel Biosciences INCTaizhou 225316China Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education/Beijing)Clinical LaboratoryPeking University Cancer Hospital&InstituteBeijing 100142China 

出 版 物：《Chinese Journal of Cancer Research》 (中国癌症研究（英文版）)

年 卷 期：2019年第31卷第6期

页      面：945-954页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by the National Science Foundation for Young Scientists of China (No. 81802735) 

主　　题：NEimFISH gastric cancer peritoneal metastasis conventional cytology intraperitoneal free cancer cells 

摘      要：Objective:To explore the intraperitoneal free cancer cell(IFCC)detection value of negative enrichment and immune fluorescence in situ hybridization(NEimFISH)on chromosomes(CEN)8/***:To verify the reliability of NEimFISH,29 gastric cancer tumors,their adjacent tissues and greater omental tissues were *** study then included 105 gastric cancer patients for *** defined patients as IFCC-positive if a signal was detected,regardless of the detailed cancer cell numbers.A comparison of clinicopathological features was conducted among IFCC *** also compared the diagnosis value and peritoneal recurrence predictive value among different detection *** comparison of IFCC number was also conducted among different ***:A cutoff of 2.5 positive cells could distinguish all benign tissue samples and 97%of malignant tissue samples in our *** to intestinal gastric cancer,patients with diffuse gastric cancer tended to have more IFCCs(6 vs.4,P=0.002).The IFCC counts were often higher in the lymphovascular invasion positive group than negative group(3 vs.1,P=0.022).All IFCC samples that were considered positive using conventional cytology were also found to be positive using *** compared to conventional cytology and paraffin pathology,NEimFISH had a higher IFCC positive rate(68.9%)and higher one-year peritoneal recurrence predictive value with area under the curve(AUC)of ***:Gastric cancer could be effectively diagnosed by *** IFCC number found using NEimFISH on CEN8/17 is closely associated with Lauren type and vascular invasion of *** is a reliable detection modality with a higher positive detection rate,higher one-year peritoneal recurrence predictive value and quantitative features for IFCC of gastric cancer.