Preparation and Charaterization of Self-assembled Nanoparticles Based on Linolenic-acid Modified Chitosan

作     者：LIU Chenguang Desai Kashappa Goud H. CHEN Xiguang Park Hyun-Jin 

作者机构：College of Life Science Ocean University of China Qingdao 266003 P.R. China Graduate School of Biotechnology Korea University Seoul 136-701 Korea 

出 版 物：《Journal of Ocean University of China》 (中国海洋大学学报（英文版）)

年 卷 期：2005年第4卷第3期

页      面：234-239页

学科分类：07[理学] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：National Natural Science Foundation of China(30370344) Korea Science and Engineering Foundation(19992-220-009-4)supported this study 

主　　题：壳聚糖 聚合浓度 荧光性 亮度 分核 白蛋白 BSA 水溶性 聚合体 

摘      要：Chitosan was modified by conjugating coupling with linolenic acid through the l-ethyl-3-( 3-dimethylami-nopropyyl) carbodiimmide (EDC)-mediated reaction. The degree of substitution 1.8% (i.e. 1.8 linolenic acid group per 100anhydroglucose units) was measured by 1H NMR. The critical aggregation concentration (CAC) of the self-aggregate of hy-drophobically modified chitosan was determined by measuring the fluorescence intensity of the pyrene as a fluorescent probe.The CAC value in phosphate-buffered saline (PBS) solution (pH7.4) was 5 × 10-2 mg mL-1. The average particle size of self-aggregates of hydrophobically modified chitosan in PBS solution (pH7.4) was 210.8 nm with a unimodal size distributionranging from 100 to 500 nm. Transmission electron microscopy (TEM) study showed that the formation of near sphericalshape nanoparticles has enough structural integrity. The loading ability of hydrophibically modified chitosan (LA-chitosan)was investigated by using bovine serum albumin (BSA) as the model. The loading capacity of self-aggregated nanoparticlesincreases (19.85% ±0.04% to 37.57% ±0.25%) with the concentration of BSA (0.1-0.5mgmL-1).