Determination of low level HBsAg in serum by microparticle enzyme immunoassay

作     者：Yu Chen Wei Wu From the First Affiliated Hospital, Zhejiang University, School of Medicine, Hangzhou 310003, China 

作者机构：First Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310003 China. chenyuheji1016@*** 

出 版 物：《Hepatobiliary & Pancreatic Diseases International》 (国际肝胆胰疾病杂志（英文版）)

年 卷 期：2002年第1卷第2期

页      面：262-264页

学科分类：1010[医学-医学技术（可授医学、理学学位）] 10[医学] 

主　　题：hepatitis B surface antigen immunoenzyme techniques 

摘      要：Objective: To investigate the distribution of the popula- tion with iow level HBsAg in serum and the characteri- stics of their HBV serologic markers. Methods: We detected HBsAg, HBeAg, anti-HBc, an- ti-HBe, and anti-HBs in the serum samples from 8089 non-hepatitis inpatients and defined the patients with HBsAg concentration equal to or less than 5 μg/L by using the AxSYM microparticle enzyme immunoassay (MEIA) system. Results: 189 patients with HBsAg equal to or Iow than 5 μg/L (2.34% of all 8089 patients and 23.16% of 816 HBsAg positive patients) included 84 (44.40%), 33 (17.5%) and 72 (38.10%) with HBsAg equal to or less than 1 μg/L, 1-2 μg/L and 2-5 μg/L respec- tively. Most of these patients were detected with posi- tive for HBsAg, anti-HBc and anti-HBe but negative for HBeAg and anti-HBs , and anti-HBc antibodies were frequently present in their sera. Conclusions: The population with low Level HBsAg in serum should not be neglected and it is important to improve the test sensitivity of HBsAg and to detect the other HBV serologic markers.