Propolis modulates vitronectin,laminin,and heparan sulfate/heparin expression during experimental burn healing

作     者：Pawe OLCZYK Katarzyna KOMOSINSKA-VASSEV Katarzyna WINSZ-SZCZOTKA Ewa M.KOZMA Grzegorz WISOWSKI Jerzy STOJKO Katarzyna KLIMEK Krystyna OLCZYK 

作者机构：Department of Clinical Chemistry and Laboratory DiagnosticsMedical University of Silesia41-200 SosnowiecPoland Center of Experimental MedicineMedical University of Silesia40-752 KatowicePoland Department of StatisticsMedical University of Silesia41-200 SosnowiecPoland 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2012年第13卷第11期

页      面：932-941页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 0905[农学-畜牧学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 0906[农学-兽医学] 10[医学] 

基　　金：Project (KNW-2-138/09) supported by the Medical University of Silesia  Poland 

主　　题：Apitherapeutic agent Silver sulfadiazine Laminin Vitronectin Heparan sulfate/heparin Wound healing 

摘      要：Objective:This study was aimed at assessing the dynamics of vitronectin (VN), laminin (LN), and heparan sulfate/heparin (HS/HP) content changes during experimental burn healing. Methods:VN, LN, and HS/HP were isolated and purified from normal and injured skin of domestic pigs, on the 3rd, 5th, 10th, 15th, and 21st days following thermal damage. The wounds were treated with apitherapeutic agent (propolis), silver sulfadiazine (SSD), physiological salt solution, and propolis vehicle. VN and LN were quantified using an immunoenzymatic assay and HS/HP was estimated by densitometric analysis. Results:Propolis treatment stimulated significant increases in VN, LN, and HS/HP contents during the initial phase of study, followed by a reduction in the estimated extracellular matrix molecules. Similar patterns, although less extreme, were observed after treatment with SSD. Conclusions:The beneficial effects of propolis on experimental wounds make it a potential apitherapeutic agent in topical burn management.